中国生物工程杂志

It is of primary importance to construct a suitable gene delivery system in the technology of transgenic animal generation and gene therapy, which can effectively mediate the exogenous genes expressed in the target cells. Vectors used for the genes transfer can be classified into virus vectors and non-virus vectors, thereinto, researches on virus vectors trend to be a hot spot in the technology of gene engineering attributing to its high efficiency of transfection and the stably expression of exogenous genes . However, because of the diversity of virus category, specificity of its structure and its complicated parasitic relationship with host organism , scientists are still working on being more familiar with its functional mechanism，life cycle and relationship with pathogenesis, which makes kinds of virus still not yet to be developed as gene delivery vector. Presently, just a few kinds of virus have been successfully reconstructed as gene delivery system, and its progress in transgenic technology will be summarized in this essay.