仙客来是一种重要的观赏花卉，为了建立其稳定的快繁增殖体系并研究体胚变异，本研究以仙客来种苗为试材，经过初培养和多次继代培养，诱导产生胚性愈伤，并建立了胚性细胞的悬浮培养体系。胚性细胞团转至无激素的培养基上，悬浮培养和固体培养均获得了大量体细胞胚，根据体胚萌发状态不同对体胚变异进行统计，固体培养基上得到10.9%的正常体胚，其体胚变异机率为89.1%，悬浮培养得到6.25%的正常体胚，变异机率为93.75%。结果表明，本研究可以为仙客来通过体胚发生进行快繁提供技术参考，体胚存在很大变异
To develop a fast and easy reproducible propagation system for Cyclamen persicum Mill. and study variation of somatic embryos, the tubers of seedlings were used as explants, and embryogenic callus were induced after cultures and subcultures. The embryogenic callus were used as inocula for establishment of suspension culture system. The regeneration of somatic embryos in high frequencies was observed after plating the cultures from solid and liquid medium on hormone-free medium.
卞福花,龚雪琴,由翠荣,曲复宁. 仙客来体细胞胚胎的发生及其变异[J]. 中国生物工程杂志, 2008, 28(专刊): 103-106.
. Somatic Embryogenesis and Variation of Somatic Embryos in Cyclamen persicum Mill. China Biotechnology, 2008, 28(专刊): 103-106.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I专刊/103
Cited
