补料发酵法批量培养磁敏感氧化亚铁硫杆菌

doi:Q93-335

中国生物工程杂志

2010, Vol. 30

Issue (06): 109-112 DOI: Q93-335

技术与方法

补料发酵法批量培养磁敏感氧化亚铁硫杆菌

刘新星^**,郭宁,梁万洁,张剑

中南大学资源加工与生物工程学院长沙 410083

Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation

LIU Xin-xing,GUO Ning,LIANG Wan-jie,ZHANG Jian

School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China

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摘要：

磁敏感氧化亚铁硫杆菌胞内可以生成磁性颗粒，探索其培养条件对趋磁细菌的研究有重要意义。利用补料发酵法，实现了磁敏感氧化亚铁硫杆菌的实验室批量培养。发酵终体积为2 L的摇瓶发酵条件下，培养40 h后一次补Fe2+（5%），使最高菌体浓度达到2.33×107 个/ml，比不补料对照提高了60.69%；同样条件下，补入9K全料则可达到2.47×107个/ml，比不补料对照提高70.34%；原子力显微镜磁扫描结果显示发酵得到的菌体有明显磁性，即胞内含有大量磁性颗粒。

关键词： 磁敏感氧化亚铁硫杆菌; 补料发酵; 原子力显微镜

Abstract:

Acidithiobacillus ferrooxidans (A.f) could synthesize intra-cellular nanometer magnetic particles. With the development of study on their culture conditions, the research of magnetotaxis bacteria will get more benefit from it. By fed-batch fermentation magnetic A.f could be batch cultured in the laboratory. In the shake-flask fermentation, the final volume of liquid was 2 L. After 40 h in the fermentation Fe2+ (5%) was added in the fermentation liquid. The max cell density could reach 2.33×107cells·ml-1. Compared with control experiments, it increased by 60.69%. At the same condition, the completion 9K culture medium was added in the fermentation liquid. The max cell density could reach 2.47×107cells·ml-1. Compared with control experiments, it increased by 70.34%. And atom force microscopy and magnetic force microscopy showed that cells in the fermentation were magnetic.

Key words: Magnetic Acidithiobacillus ferrooxidans Fed-batch fermentation Atom force microscopy

收稿日期: 2010-02-01 出版日期: 2010-06-12

基金资助:

国家“973”计划（2004CB619201）、国家自然科学基金（50774102）资助项目

通讯作者: 刘新星 E-mail: xxlcsu@126.com

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引用本文:

刘新星郭宁梁万洁张剑. 补料发酵法批量培养磁敏感氧化亚铁硫杆菌[J]. 中国生物工程杂志, 2010, 30(06): 109-112.

LIU Xin-Xing, GUO Ning, LIANG Mo-Ji, ZHANG Jian. Batch Culture of Magnetic Acidthiobacillus ferrooxidans by Fed-batch Fermentation. China Biotechnology, 2010, 30(06): 109-112.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/Q93-335 或 https://manu60.magtech.com.cn/biotech/CN/Y2010/V30/I06/109

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