研究报告 |
|
|
|
|
Tau蛋白核心片段306~378的异源表达、纯化及聚集特性验证* |
位薇,常保根,王英,路福平(),刘夫锋() |
工业发酵微生物教育部重点实验室 天津市工业微生物重点实验室 工业酶国家工程实验室天津科技大学生物工程学院 天津 300457 |
|
Heterologous Expression, Purification and Aggregation Characterization of Tau Core Fragment 306-378 |
WEI Wei,CHANG Bao-gen,WANG Ying,LU Fu-ping(),LIU Fu-feng() |
Key Laboratory of the Ministry of Education of Industrial Fermentation Microorganisms, Tianjin Key Laboratory of Industrial Microbiology, National Engineering Laboratory of Industrial Enzymes, School of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457, China |
引用本文:
位薇,常保根,王英,路福平,刘夫锋. Tau蛋白核心片段306~378的异源表达、纯化及聚集特性验证*[J]. 中国生物工程杂志, 2020, 40(5): 22-29.
WEI Wei,CHANG Bao-gen,WANG Ying,LU Fu-ping,LIU Fu-feng. Heterologous Expression, Purification and Aggregation Characterization of Tau Core Fragment 306-378. China Biotechnology, 2020, 40(5): 22-29.
链接本文:
https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.1911035
或
https://manu60.magtech.com.cn/biotech/CN/Y2020/V40/I5/22
|
[1] |
Fitzpatrick A, Falcon B, He S , et al. Cryo-EM structures of Tau filaments from Alzheimer's disease brain. Nature, 2017,547(7662):185-190.
|
[2] |
Hardy J . Alzheimer's disease: the amyloid cascade hypothesis: an update and reappraisal. Journal of Alzheimers Disease, 2006,9(S3):151-153.
|
[3] |
Rossor M N . Molecular pathology of Alzheimer's disease. Journal of Neurology Neurosurgery and Psychiatry, 1993,56(6):583-586.
|
[4] |
Yankner B A, Duffy L K, Kirschner D A . Neurotrophic and neurotoxic effects of amyloid beta protein: reversal by tachykinin neuropeptides. Science, 1990,250(4978):279-282.
|
[5] |
Wang Y, Mandelkow E . Tau in physiology and pathology. Nature Reviews Neuroscience, 2016,17(1):5-21.
|
[6] |
Liu F F, Dong X Y, He L Z , et al. Molecular insight into conformational transition of amyloid beta-peptide 42 inhibited by (-)-epigallocatechin-3-gallate probed by molecular simulations. Journal of Physical Chemistry B, 2011,115(41):11879-11887.
|
[7] |
Kametani F, Hasegawa M . Reconsideration of amyloid hypothesis and Tau hypothesis in Alzheimer's disease. Frontiers in Neuroscience, 2018,12(25):1-11.
|
[8] |
Brier M R, Gordon B, Friedrichsen K , et al. Tau and abeta imaging, CSF measures, and cognition in Alzheimer's disease. Science Translational Medicine, 2016,8(338):338ra366.
|
[9] |
Shammas S L, Garcia G A, Kumar S , et al. A mechanistic model of Tau amyloid aggregation based on direct observation of oligomers. Nature Communications, 2015,6(1):1-10.
|
[10] |
Mandelkow E M, Mandelkow E . Tau protein and Alzheimer's disease. Neurobiology of Aging, 1994,15(1):85-86.
|
[11] |
Rademakers R, Cruts M, van Broeckhoven C . The role of tau (MAPT) in frontotemporal dementia and related tauopathies. Human Mutation, 2010,24(4):277-295.
|
[12] |
Verwilst P, Kim H S, Kim S , et al. Shedding light on Tau protein aggregation: the progress in developing highly selective fluorophores. Chemical Society Reviews, 2018,47(7):2249-2265.
|
[13] |
Seidler P M, Boyer D R, Rodriguez J A , et al. Structure-based inhibitors of Tau aggregation. Nature Chemistry, 2018,10(2):170-176.
|
[14] |
Bratus S, Pak I . Structure, microtubule interactions, and paired helical filament aggregation by Tau mutants of frontotemporal dementias. Biochemistry, 2000,39(38):11714-11721.
doi: 10.1021/bi000850r
|
[15] |
Hasegawa M, Smith M J, Goedert M . Tau proteins with FTDP-17 mutations have a reduced ability to promote microtubule assembly. Febs Letters, 1998,437(3):207-210.
|
[16] |
Karikari T K, Turner A, Stass R , et al. Expression and purification of Tau protein and its frontotemporal dementia variants using a cleavable histidine tag. Protein Expression and Purification, 2016,130:44-54.
|
[17] |
Michel C H, Kumar S, Pinotsi D , et al. Extracellular monomeric Tau protein is sufficient to initiate the spread of Tau protein pathology. Journal of Biological Chemistry, 2013,289(2):956-967.
doi: 10.1074/jbc.M113.515445
|
[18] |
Yao T M, Tomoo K, Ishida T , et al. Aggregation analysis of the microtubule binding domain in Tau protein by spectroscopic methods. Journal of Biochemistry, 2003,134(1):91-99.
|
[19] |
Barghorn S, Davies P, Mandelkow E . Tau paired helical filaments from Alzheimer's disease brain and assembled in vitro are based on β-structure in the core domain. Biochemistry, 2004,43(6):1694-1703.
|
[20] |
Jia L G, Wang W J, Shang J Z , et al. Highly efficient soluble expression, purification and characterization of recombinant Aβ42 from Escherichia coli. Rsc Advances, 2018,8(33):18434-18441.
|
[21] |
Du W J, Guo J J, Gao M T , et al. Brazilin inhibits amyloid β-protein fibrillogenesis, remodels amyloid fibrils and reduces amyloid cytotoxicity. Scientific Reports, 2015,5(1):1-10.
|
[22] |
Jia L G, Wang W J, Sang J C , et al. Amyloidogenicity and cytotoxicity of a recombinant C-terminal His-Tagged Aβ. Acs Chemical Neuroscience, 2019,10(3):1251-1262.
|
[23] |
Li S, Liu F F, Yu L L , et al. Dual effect of thioflavin T on the nucleation kinetics of amyloid β-protein 40. Acta Physico-Chemica Sinica, 2016,32(6):1391-1396.
|
[24] |
Kundel F, De S, Flagmeier P , et al. Hsp70 inhibits the nucleation and elongation of Tau and sequesters Tau aggregates with high affinity. Acs Chemical Biology, 2018,13(3):636-646.
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|