技术与方法 |
|
|
|
|
重组sTNFR1蛋白包涵体洗涤方法的研究 |
罗莉1, 秦娇荣2, 王明蓉2 |
1. 四川抗菌素工业研究所 成都 610052; 2. 成都生物制品研究所有限责任公司 成都 610023 |
|
Study on Washing Method of Recombinant sTNFR1IBs |
LUO Li1, Qin2, Jiao-rong2 |
1. Sichuan Industrial Institute of Antibiotics, Chengdu 610052, China; 2. Chengdu Institute of Biological Products Co., Ltd., Chengdu 610023, China |
[1] White C B, Chen Q, Kenyon G L,et al.A novel activity of OmpT. Proteolysis under extreme denaturing conditions.J Biol Chem, 1995, 270(22): 12990-12994. [2] Clark E D.Protein refolding for industrial processes.Curr Opin Biotechnol, 2001, 12(2): 202-207. [3] Roussel G, Perpete E A, Matagne A,et al.Towards a universal method for protein refolding: The trimeric beta barrel membrane Omp2a as a test case.Biotechnol Bioeng, 2013, 110(2): 417-423. [4] Das D, Jacobs F, Feldmann H,et al.Differential expression of the Ebola virus GP(1,2) protein and its fragments in E. coli.Protein Expr Purif, 2007, 54(1): 117-125. [5] Jevevar S, Gaberc-Porekar V, Fonda I,et al.Production of nonclassical inclusion bodies from which correctly folded protein can be extracted.Biotechnol Progr, 2005, 21(2): 632-639. [6] García-Fruitós E, González-Montalbán N, Morell M,et al.Aggregation as bacterial inclusion bodies does not imply inactivation of enzymes and fluorescent proteins.Microb Cell Fact, 2005, 4(1): 27-32. [7] Peternel Š, Bele M, Gaberc-Porekar V,et al.Inclusion bodies contraction with implications in biotechnology.Acta Chim Slov, 2008, 55(3): 608-612. [8] Carvajal P, Gibert J, Campos N,et al.Activity of maize transglutaminase overexpressed in Escherichia coli inclusion bodies:an alternative to protein refolding.Biotechnol Progr, 2011, 27(1): 232-240. [9] Tsumoto K, Umetsu M, Kumagai I,et al.Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine.Biochem Biophys Res Commun, 2003, 312(4): 1383-1386. [10] Tsuji I, Mastui H, Ito T,et al.L-cysteine-enhanced renaturation of bioactive soluble tumor necrosis factor ligand family member LIGHT from inclusion bodies in Escherichia coli.Protein Expres Purif, 2011, 80(2): 239-245. [11] Peternel Š, Komel R.Isolation of biologically active nanomaterial(inclusion bodies) from bacterial cells.Microbial cell factories, 2010, 9(1): 66-81. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|