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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2013, Vol. 33 Issue (8): 56-60    
研究报告     
混合油脂补料发酵提高多杀菌素的产量
王美玲, 薛超友, 赵方龙, 卢文玉
天津大学化工学院 天津 300072
Enhanced Production of Spinosad by Fed-batch Fermentation
WANG Mei-ling, XUE Chao-you, ZHAO Fang-long, LU Wen-yu
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
 全文: PDF(1082 KB)   HTML
摘要:

通过补加植物油(菜籽油、玉米油、豆油)、油酸甲酯、玉米浆,考察其对多杀菌素发酵产量的影响。结果显示,在96h补加3%的混合油脂(油酸甲酯:菜籽油=2:1),同时在96h和144h分别补加1%的玉米浆可使多杀菌素的产量提高2.11倍,发酵单位达到804.59 mg/L,为实现工业化生产奠定基础。

关键词: 补料分批发酵多杀菌素混合油脂刺糖多孢菌    
Abstract:

The effect of the addition of corn oil, rapeseed oil, soybean oil, methyl oleate, the mixture of rapeseed oil and metyl oleate on the production of spinosad by Saccharopolyspora spinosa LU 104 was studied. The results indicated that the yield of spinosad was increased 2.11-fold up to 804.59 mg/L when adding 3% mixture oil (rapeseed oil:metyl oleate=1:2) at 96 h and 1% corn steep liquor at 96 h and 144 h.

Key words: Fed-batch fermentation    Spinosad    Mixed lipid    Saccharopolyspora spinosa
收稿日期: 2013-04-15 出版日期: 2013-08-25
ZTFLH:  Q819  
基金资助:

国家自然科学基金(21076148);天津市科技支撑计划重点项目(11ZCKFSY0100)资助项目

通讯作者: 卢文玉wenyulu@tju.edu.cn     E-mail: wenyulu@tju.edu.cn
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引用本文:

王美玲, 薛超友, 赵方龙, 卢文玉. 混合油脂补料发酵提高多杀菌素的产量[J]. 中国生物工程杂志, 2013, 33(8): 56-60.

WANG Mei-ling, XUE Chao-you, ZHAO Fang-long, LU Wen-yu. Enhanced Production of Spinosad by Fed-batch Fermentation. China Biotechnology, 2013, 33(8): 56-60.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I8/56

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