研究报告 |
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启动子和质粒对酵母细胞青蒿二烯产量的影响 |
王思佳, 丁明珠, 元英进 |
系统生物工程教育部重点实验室 天津大学化工学院制药工程系 天津 300072 |
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The Impact of Promoters and Vectors on the Amorphadiene Production in Yeast Cells |
WANG Si-jia, DING Ming-zhu, YUAN Ying-jin |
Key Laboratory of Systems Biology, Ministry of Education;Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
[1] 孔建强,程克棣,王丽娜,等. HMG-CoA还原酶和FPP合酶基因拷贝数对紫穗槐-4,11-二烯酵母工程菌产量的影响. 药学学报,2007,42(12):1314-1319. Kong J Q, Cheng K D, Wang L N, et al. Increase of copy number of HMG-CoA reductase and FPP synthase genes improves the amorpha-4,11-diene production in engineered yeast. Acta Pharmaceutica Sinica,2007,42(12):1314-1319. [2] 刘夺,杜瑾,赵广荣,等.合成生物学在医药及能源领域的应用. 化工学报,2011,62(9):2391-2397. Liu D, Du J, Zhao G R, et al. Applications of synthetic biology in medicine and energy.CIESC Journal,2011,62(9):2391-2397. [3] 孔建强,黄勇,沈君豪,等. 紫穗槐-4,11-二烯合酶及其代谢工程研究进展. 药学学报,2009,44 (12):1320-1327. Kong J Q, Huang Y, Shen J H, et al. Recent advances in the study of amorpha-4,11-diene synthase and its metabolic engineering . Acta Pharmaceutica Sinica,2009,44 (12):1320-1327. [4] Martin V J J, Pitera D J, Withers S T, et al. Engineering a mevalonale pathway in Escherichia coli for production of terpenoids.Nat Biotechnol,2003,21(7):796-803. [5] Newman J D, Marshall J, Chang M, et al. High-level production of amorpha4,11-diene in a two-phase partitioning bioreactor of metabolically engineered Escherichia coli. Biotech Bioeng,2006,95(4):684-691. [6] Lindahl A L, Olsson M E, Mercke P, et al.Production of the artemisinin precursor amorpha-4,11-diene by engineered Saccharomyces cerevisiae.Biokchnol Lett,2006,28(8):571-580. [7] Ro D K, Paradise E M, Ouellet M, et al. Production of the antimalarial drug precursor artemisinic acid in engineered yeast.Nature,2006,440(13):940-943. [8] Alper H, Fischer C, Nevoigt E, et al. Tuning genetic control through promoter engineering. PNAS,2005,102(36):12678-12683. [9] Nevoigt E, Kohnke J, Fischer C R, et al.Engineering of promoter replacement cassettes for fine-tuning of gene expression in Saccharomyces cerevisiae. Appl Environ Microbiol,2006,72(8):5266-5273. [10] Chenfeng L, Jeffries T. Shuffling of promoters for multiple genes to optimize xylose fermentation in an engineered Saccharomyces cerevisiae strain.Appl Environ Microbiol,2007 ,73(19):6072-6077. [11] Lee T I, Rinaldi N J, Robert F, et al. Transcriptional regulatory networks in Saccharomyces cerevisiae.Science,2002,298:799-804. [12] 孔建强,沈君豪,黄勇,等.酵母工程菌制备紫穗槐-4,11-二烯的研究. 药学学报,2009,44 (11):1297-1303. Kong J Q, Shen J H, HuangY, et al. Production of amorpha-4,11-diene in engineered yeasts. Acta Pharmaceutica Sinica,2009,44 (11):1297-1303. [13] Adams A.酵母遗传学实验方法指南.北京:科学出版社,2000. Adams A. Methods in Yeast Genetics. Beijing:Science Press,2000. [14] Gietz R D, Schiestl R H, Willems A R, et al. Studies on the transformation of intact yeast cells by the LiAc/ss-DNA/PEG procedure . Yeast,1995,11(4):355-360. [15] Wallaart T E, Bouwmeester H J, Hille J, et al. Amorpha-4,11-diene synthase:cloning and functional expression of a key enzyme in the biosynthetic pathway of the novel antimalarial drug artemisinin. Planta,2001,212:460-465. [16] Ro D K, Ouellet M, Paradise E M, et al. Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid. BMC Biotechnology, 2008,8:83. [17] Mumberg D, Muller R, Funk M. Regulatable promoters of Saccharomyces cerevisiae:comparison of transcriptional activity and their use for heterologous expression.Nucleic Acids Research,1994,22(25):5767-5768. [18] Ohto C, Muramatsu M, Obata S, et al. Overexpression of the gene encoding HMG-CoA reductase in Saccharomyces cerevisiae for production of prenyl alcohols. Appl Microbiol Biotechnol ,2009,82:837-845. [19] Kong J Q, Wang W, Wang L N. et al. The improvement of amorpha-4,11-diene production by a yeast-conform variant. Journal of Applie Microbiology,2009,106:941-951. [20] 贾云婧,赵鹃,丁明珠,等. 青蒿二烯功能模块与酵母底盘的适配性研究.高校化学学报,2014,2. Jia Y J, Zhao J, Ding M Z. et al. Fitness of amorphadiene production functional modules and yeast chassis. Chiemical Journal of Chinese Universities,2014,2. [21] Muntendam R, Melillo E, Ryden A, et al. Perspectives and limits of engineering the isoprenoid metabolism in heterologous hosts.Appl Microbiol Biotechnol,2009,84:1003-1019. [22] Engelsa B, Dahmb P, Jennewein S. Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards taxol(paclitaxel) production. Metabolic Engineering,2008,10:201-206. [23] Dai Z B, Liu Y, Huang L Q, et al. Production of miltiradiene by metabolically engineered Saccharomyces cerevisiae. Biotechnology and Bioengineering, 2012,109(11):2845-2853. |
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