米根霉发酵产富马酸的最适替代中和剂及pH调控策略研究

中国生物工程杂志

2013, Vol. 33

Issue (4): 85-91

研究报告

米根霉发酵产富马酸的最适替代中和剂及pH调控策略研究

陈晨, 邰超, 李霜

南京工业大学生物与制药工程学院材料化学工程国家重点实验室南京 210009

Search for Optimum Substitutive Neutralizing Agent and pH Control Strategy in Fumaric Acid Fermentation by Rhizopus oryzae

CHEN Chen, TAI Chao, LI Shuang

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China

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摘要： 针对米根霉发酵产富马酸使用的不同中和剂(CaCO₃,Na₂CO₃,NH₃·H₂O,NaOH)进行了研究,结果表明发酵过程中使用Na₂CO₃作为中和剂时富马酸产率和生产强度最接近传统中和剂CaCO₃。此后考察了不同pH值(3.5,4.5,5.5和6.5)对Na₂CO₃作为中和剂的富马酸发酵过程的影响。基于对3个动力学参数的分析,提出了一个旨在同时获得富马酸高产物浓度、高产率和高生产强度的双阶段pH调控策略,在初始的24 h内pH控制在5.5,然后将pH调到4.5直至发酵结束。最终富马酸的终浓度达到40.5 g/L,产率为0.55 g/g,生产强度为0.61 g/L/h,比恒定pH时的最优结果分别提高了8.3%,10.0%和17.3%,其中生产强度甚至比使用CaCO₃时还高了3.4%。故以Na₂CO₃作为中和剂,采用双阶段pH调控策略具有降低能耗和简化下游步骤的优势,可以成功取代CaCO₃。

关键词： 富马酸; 米根霉; 中和剂; 动力学分析; 双阶段pH调控策略

Abstract: Four different neutralizing agents (CaCO₃, Na₂CO₃, NH₃稨₂O, NaOH) were chosen to examine the effects of neutralizing agents on fumaric acid fermentation by Rhizopus oryzae ME-F12 which is the mutant of R. oryzae ATCC 20344. It was found that fumaric acid yield and productivity in the fermentation using Na₂CO₃ as neutralizing agent were the closest to which in the traditional CaCO₃ case. Then the effects of different pH values (3.5, 4.5, 5.5, and 6.5) on fumaric acid fermentation using Na₂CO₃ as neutralizing agent were investigated. Based on the analysis of three kinetic parameters, a two-stage pH control strategy, aimed at achieving high concentration, high yield and high productivity of fumaric acid simultaneously, was proposed. pH was controlled at 5.5 at the first 24 h, and then switched to 4.5 till the end of the fermentation. Finally, the maximum concentration of fumaric acid reached 40.5 g/L with the yield of 0.55 g/g and the productivity of 0.61 g/L/h, which were 8.3%, 10.0% and 17.3% higher than the best result of constant pH control process. Its productivity was even 3.4% higher than which in CaCO₃ case. With the internal advantages of reducing power consumption and simplifying downstream processing, using Na₂CO₃ as neutralizing agent under two-stage pH control strategy successfully take place of CaCO₃.

Key words: Fumaric acid Rhizopus oryzae Neutralizing agents Kinetic analysis Two-stage pH control strategy

收稿日期: 2012-12-13 出版日期: 2013-04-25

ZTFLH:

Q819

基金资助: 国家"863"计划(2011AA02A206);国家自然科学基金(21076104)资助项目

通讯作者: 李霜 E-mail: lishuang@njut.edu.cn

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引用本文:

陈晨, 邰超, 李霜. 米根霉发酵产富马酸的最适替代中和剂及pH调控策略研究[J]. 中国生物工程杂志, 2013, 33(4): 85-91.

CHEN Chen, TAI Chao, LI Shuang. Search for Optimum Substitutive Neutralizing Agent and pH Control Strategy in Fumaric Acid Fermentation by Rhizopus oryzae. China Biotechnology, 2013, 33(4): 85-91.

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I4/85

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