新型γ-聚谷氨酸自组装纳米胶束的制备及用于蛋白载体的研究

中国生物工程杂志

2013, Vol. 33

Issue (4): 101-105

技术与方法

新型γ-聚谷氨酸自组装纳米胶束的制备及用于蛋白载体的研究

陈宽婷¹, 姚俊¹, 阮文辉², 魏钦俊¹, 鲁雅洁¹, 曹新¹

1. 南京医科大学基础医学院南京 210029;
2. 山西省医药与生命科学研究院太原 030001

Preparation of a Novel Self-assembly Nanoparticle Based on Amphiphilic γ-Polyglutamic Acid Derivatives as a Protein Carrier

CHEN Kuan-ting¹, YAO Jun¹, RUAN Wen-hui², WEI Qin-jun¹, LU Ya-jie¹, CAO Xin¹

1. Department of Biotechnology, School of Basic Medical Science, Nanjing Medical University, Nanjing 210029, China;
2. Shanxi Institute of Medicine and Life Science, Taiyuan 030001, China

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摘要： 对水溶性的γ-聚谷氨酸(γ-PGA)进行了接枝改性,合成了两亲性γ-聚谷氨酸(γ-PGA)接枝衍生物,采用超声探头法制备胆甾醇基γ-PGA自组装胶束,并以卵清蛋白(OVA)作为模型蛋白,研究其载药和释药性能。结果表明,制备的两亲性胆甾醇基γ-PGA自组装胶束平均粒径为299.6+27.3nm,粒径的多分散系数较窄(0.17),且具有较低的细胞毒性;其疏水核-亲水壳的纳米微结构对蛋白药物显示了良好载药性能,对OVA载药量可达118.8μg/mg,包封率33.5%;体外释药结果显示,负载OVA的甾醇基γ-PGA自组装胶束能延缓蛋白的释放,释药速率与介质pH密切相关。

关键词： γ-聚谷氨酸; 接枝共聚物; 自组装纳米胶束; 药物载体

Abstract: A novel amphiphilic graft copolymer composed of γ-polyglutamic acid (γ-PGA) as the hydrophilic backbone and cholesterol as the hydrophobic segment was synthesized. The cholesterol-bearing γ-PGA (γ-PGA-Graft-CH) was used to form self-assembly nanoparticles (γ-PGA-Graft-CH NPs) with an inner hydrophobic core and an outer hydrophilic shell via the ultrasonic probe method. The obtained nanoparticles showed low cytotoxicity and a narrow size distribution (PDI=0.17) with a mean diameter 299.6+5.4 nm. OVA-loading γ-PGA-Graft-CH NPs was also successfully prepared, with drug loading content of 118.8μg/mg, and entrapment efficiency of 33.5%. The experimental results also showed that OVA continuously released from γ-PGA-Graft-CH NPs in the phosphate buffered saline (PBS) solutions,and its release was sensitive to the pH of the release medium.

Key words: γ-Polyglutamic acid Graft copolymer Self-assembly nanoparticles Drug carrier

收稿日期: 2012-10-18 出版日期: 2013-04-25

ZTFLH:

Q819

基金资助: 山西省科技攻关项目(20110321081-01,20120313016-6);江苏高校优势学科建设工程资助项目

通讯作者: 曹新 E-mail: biotechnol@163.com

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引用本文:

陈宽婷, 姚俊, 阮文辉, 魏钦俊, 鲁雅洁, 曹新. 新型γ-聚谷氨酸自组装纳米胶束的制备及用于蛋白载体的研究[J]. 中国生物工程杂志, 2013, 33(4): 101-105.

CHEN Kuan-ting, YAO Jun, RUAN Wen-hui, WEI Qin-jun, LU Ya-jie, CAO Xin. Preparation of a Novel Self-assembly Nanoparticle Based on Amphiphilic γ-Polyglutamic Acid Derivatives as a Protein Carrier. China Biotechnology, 2013, 33(4): 101-105.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I4/101

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