碳源、氮源对异养单针藻<em>Monoraphidium </em>sp. FXY-10油脂积累和脂肪酸组成的影响

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中国生物工程杂志 ›› 2013, Vol. 33 ›› Issue (2) : 59-64.

研究报告

碳源、氮源对异养单针藻Monoraphidium sp. FXY-10油脂积累和脂肪酸组成的影响

黄力, 贺赐安, 赵鹏, 余旭亚

作者信息 +

Effect of Carbon, Nitrogen Sources on Lipid Accumulation and Fatty Acid Composition of Monoraphidium sp. FXY-10 under Heterotrophic Cultivation

HUANG Li, HE Ci-an, ZHAO Peng, YU Xu-ya

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摘要

研究了碳源与氮源对单针藻Monoraphidium sp. FXY-10异养培养的影响。以BG-11为基础培养基,通过添加不同类型、浓度梯度碳源和氮源,比较分析微藻生物量、油脂积累以及脂肪酸组成。结果表明,以葡萄糖作碳源,硝酸钠为氮源,微藻细胞积累的油脂是理想的生物柴油制备原料。硝酸钠浓度分别为1.00、3.00和5.00 g/L时,对油脂产量影响不显著(P>0.05)。葡萄糖浓度为10.00 g/L,硝酸钠为氮源油脂产量达到实验最高值0.84 g/L,其油脂脂肪酸组成主要由C16:0和C18:1等短链饱和脂肪酸和单不饱和脂肪酸组成,不饱和度值(DU)为61.98,相对偏低。

Abstract

Effect of carbon and nitrogen sources on heterotrophic cultivation of microalgae Monoraphidium sp. FXY-10 is studied. The biomass, oil accumulation and fatty acid composition of microalgae cell are analysed comparatively by selecting various types and setting different concentrations of carbon and nitrogen sources with BG-11 as basic medium. The results show that microalgae lipid accumulating with glucose as carbon source and sodium nitrate as nitrogen source is an ideal candidate for biodiesel production. The lipid production is not significantly impacted (P>0.05) by different sodium nitrate concentration which was 1.00, 3.00 and 5.00 g/L, respectively. Moreover, when glucose concentration is 10.00 g/L using sodium nitrate as nitrogen source, lipid production can be obtained the highest experiment value 0.84 g/L. And the fatty acid of microalgae lipid is mainly composed of short chain saturated and monounsaturated fatty acids, such as C16:0 and C18:1. So they result a lower degree of unsaturation (DU) value 61.98.

导出引用

黄力, 贺赐安, 赵鹏, 余旭亚. 碳源、氮源对异养单针藻Monoraphidium sp. FXY-10油脂积累和脂肪酸组成的影响[J]. 中国生物工程杂志, 2013, 33(2): 59-64

HUANG Li, HE Ci-an, ZHAO Peng, YU Xu-ya. Effect of Carbon, Nitrogen Sources on Lipid Accumulation and Fatty Acid Composition of Monoraphidium sp. FXY-10 under Heterotrophic Cultivation[J]. China Biotechnology, 2013, 33(2): 59-64

中图分类号： Q819

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