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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2013, Vol. 33 Issue (12): 97-104    
技术与方法     
响应面法优化棘孢木霉产厚垣孢子发酵工艺
武为平1, 陈捷2, 李雅乾2, 陈立杰1, 段玉玺1
1. 沈阳农业大学植物保护学院北方线虫研究所 沈阳 110866;
2. 上海交通大学农业与生物学院农业部都市农业(南方)重点实验室 上海 200240
Optimization of Fermentation Process for Chlamydospores of Trichoderma asperellum by Response Surface Methodology
WU Wei-ping1, CHEN Jie2, LI Ya-qian2, CHEN Li-jie1, DUAN Yu-xi1
1. Nematology Institute of Northern China, School of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China;
2. Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, School of Agriculture & Biology, Shanghai Jiao Tong University, Shanghai 200240, China
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摘要：

采用响应面法（Box-Benhnken Design）对影响棘孢木霉（Trichoderma asperellum）ZJSX5003菌株产厚垣孢子的4个主要因素（黄豆饼粉-玉米粉用量，甘油添加量，装液量，初始pH）进行了研究。通过对试验数据进行方差分析考察了各因素及其交互作用对厚垣孢子产量的影响；进行回归分析并建立基于统计学的回归方程，计算获得最佳发酵条件：大豆饼粉-玉米粉用量33.25g/L、甘油添加量8.86ml/L、装液量99.35ml/500ml、初始pH值3.26，此时预测的厚垣孢子产量为9.56×107个/ml。在此条件下，实际测得厚垣孢子产量为9.84×107个/ml，达到理论预测值的97.07%，且比优化前提高了69.07%，进一步采用10L发酵罐进行适用性验证，厚垣孢子产量达到1.75×108个/ml，比摇瓶发酵的产量高，为木霉菌厚垣孢子菌剂开发提供了理论依据。
关键词： 响应面法BBD设计;  棘孢木霉菌;  厚垣孢子;  发酵工艺;  优化    
Abstract:

The response surface methodology (Box-Benhnken Design) was applied to investigate the four major factors (the concentration of soybean cake powder and maize powder used in medium, the volume of glycerol addition added to the medium, loading volume of liquid medium, initial pH value of medium) that have importent effect on the production of chlamydospores of Trichoderma asperellum ZJSX5003. By variance analysis on the experiment results, the influences of the the four factors and their interactions on the production of chlamydospores was investigated. By performing regression analysis and establishing regression equation based on statistics, the optimal fermentation condition was obtained, that as follow:the concentration of soybean cake powder and maize powder used in medium 33.25g /L, glycerol 8.86ml/L, each 500ml bottles contains 99.35ml liquid medium, initial pH 3.26. Under the optimal conditions,the yield of chlamydospore is expected to be 9.56×107 spores/ml, theoretically. The practical yield of chlamydospores approached 9.84×107spores/ml, reached 97.07% of the expected value, increased by 69.07% compared with pre-optimization. In order to test its applicability, used a 10L fermentation tank under the optimal condition, the yield of chlamydospore reached up to 1.75×108spores/ml,which is better than shake flask fermentation. Further researches of developing microbial inoculum of Trichoderma chlamydospore could be conducted base on this research.
Key words: BBD design of response surface methodology    Trichoderma asperellum    Chlamydospores    Fermentation process    Optimize
收稿日期: 2013-10-08 出版日期: 2013-12-25
ZTFLH:  Q819  
基金资助:

国家玉米产业技术体系（GARS-02）；农业部948项目（2011-G4）；教育部高等学校博士学科点专项科研基金（20120073120070）资助项目
通讯作者: 李雅乾，E-mail：lauren@sjtu.edu.cn；段玉玺，E-mail：duanyx6407@163.com     E-mail: lauren@sjtu.edu.cn；duanyx6407@163.com
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引用本文:

武为平, 陈捷, 李雅乾, 陈立杰, 段玉玺. 响应面法优化棘孢木霉产厚垣孢子发酵工艺[J]. 中国生物工程杂志, 2013, 33(12): 97-104.

WU Wei-ping, CHEN Jie, LI Ya-qian, CHEN Li-jie, DUAN Yu-xi. Optimization of Fermentation Process for Chlamydospores of Trichoderma asperellum by Response Surface Methodology. China Biotechnology, 2013, 33(12): 97-104.

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https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I12/97

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