发酵罐中蔗糖流加控制和pH调控对乳酸链球菌素产量的影响

中国生物工程杂志

2013, Vol. 33

Issue (12): 45-50

研究报告

发酵罐中蔗糖流加控制和pH调控对乳酸链球菌素产量的影响

汪蕊¹, 杜超¹, 左芳雷¹, 陈尚武^1,2

1. 中国农业大学食品科学与营养工程学院食品科学与工程系北京 100083;
2. 北京市科学技术协会院士专家工作站保罗生物园北京 101113

Effects of Sucrose Feeding Rate and pH Control on Nisin Production in Fermentor

WANG Rui¹, DU Chao¹, ZUO Fang-lei¹, CHEN Shang-wu^1,2

1. Department of Food Science and Engineering, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China;
2. Academician Expert Workstation of Beijing Municipal Science and Technology Association, Polo Biology Science Park, Beijing 101113, China

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摘要： 主要研究了乳酸乳球菌发酵过程中调控pH和补加蔗糖对乳酸乳球菌发酵生产及其发酵产物乳酸链球菌素效价的影响，通过将pH恒定为乳酸链球菌素产生的最佳值6.8，与不经过pH调控的乳酸乳球菌发酵生产的乳酸链球菌素效价对比，效价提高了1.2倍。分别选用2g/L·h和4g/L·h的蔗糖流加速度，当按4g/L·h恒速流加蔗糖溶液时，乳酸链球菌素效价提高约50.5%。

关键词： 乳酸乳球菌; pH调控; 蔗糖流加; 乳酸链球菌素

Abstract: Effects of the pH control and sucrose feeding rate during the fermentation period on the fermentation of Lactococcus lactis and the titer of nisin were mainly studied. Through the comparison with the nisin produced by Lactococcus lactis which not adjust the fermentation condition pH, it is found that titer of nisin yeild 1.2 times higher than that nisin without pH asjustment which adjust the fermentation condition pH to the optimum value of 6.8. The sucrose feeding rate was designed at 2 and 4 g/L·h, respectively, nisin titer increase about 50.5% by constant addition of sucrose at a feeding rate of 4 g/L·h.

Key words: Lactococcus lactis pH control Sucrose feeding Nisin

收稿日期: 2013-06-25 出版日期: 2013-12-25

ZTFLH:

Q935

基金资助: 国家自然科学基金资助项目（31071507）

通讯作者: 陈尚武，E-mail：swchen@cau.edu.cn E-mail: swchen@cau.edu.cn

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引用本文:

汪蕊, 杜超, 左芳雷, 陈尚武. 发酵罐中蔗糖流加控制和pH调控对乳酸链球菌素产量的影响[J]. 中国生物工程杂志, 2013, 33(12): 45-50.

WANG Rui, DU Chao, ZUO Fang-lei, CHEN Shang-wu. Effects of Sucrose Feeding Rate and pH Control on Nisin Production in Fermentor. China Biotechnology, 2013, 33(12): 45-50.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I12/45

[1] Cheigh C I, Park H, Choi H J, et al. Enhanced nisin production by increasing genes involved in nisin Z biosynthesis in Lactococcus lactis subsp. lactis A164. Biotechnology Letters, 2005, 27(3): 155-160.
[2] Vuyst L. Nutritional factors affecting nisin production by Lactococcus lactis subsp.lactis NIZO22186 in a synthetic medium. Journal of Applied Microbiology, 1995, 78(1): 28-33.
[3] Kim W S. Nisin production by Lactococcus lactis using two-phase batch culture. Letters in Applied Microbiology, 1997, 25(3): 169-171.
[4] De Vos W M, Mulders J W, Siezen R J, et al. Properties of nisin Z and distribution of its gene, nis Z, in Lactococcus lactis. Applied and Environmental Microbiology, 1993, 59(1): 213-218.
[5] Laridi R, Kheadr E E, Benech R O, et al. Liposome encapsulated nisin Z: optimization, stability and release during milk fermentation. International Dairy Journal, 2003, 13(4): 325-336.
[6] Parente E, Ricciardi A. Production, recovery and purification of bacteriocins from lactic acid bacteria. Applied Microbiology and Biotechnology, 1999, 52(5): 628-638.
[7] De Vuyst L, Vandamme E J. Influence of the carbon source on nisin production in Lactococcus lactis subsp. lactis batch fermentations. Journal of General Microbiology, 1992, 138(3): 571-578.
[8] Steele J L, McKAY L L. Partial characterization of the genetic basis for sucrose metabolism and nisin production in Streptococcus lactis. Applied and Environmental Microbiology, 1986, 51(1): 57-64.
[9] Callewaert R, De Vuyst L. Bacteriocin production with Lactobacillus amylovorus DCE 471 is improved and stabilized by fed-batch fermentation. Applied and Environmental Microbiology, 2000, 66(2): 606-613.
[10] Van't Hul J S, Gibbons W R. Neutralization/recovery of lactic acid from Lactococcus lactis: effects on biomass, lactic acid, and nisin production. World Journal of Microbiology and Biotechnology, 1997, 13(5): 527-532.
[11] 焦世耀, 张兰威, 李春. 管碟法测定 nisin 效价的改进. 食品科学, 2005, 26(7): 175-176. Jiao S Y, Zhang L W, Li C.Improved Oxford assay on estimation of nisin.Food Science, 2005, 26(7):175-176.
[12] 伊守亮, 肖林, 顾正华, 等. 管碟法测定 Nisin 效价. 无锡轻工大学学报, 2004, 23(4): 41-45. Yi S L, Xiao L, Gu Z H, et al.Determination of nisin activity by oxford plate assay system.Journal of Wuxi Univerisity of Light Industry, 2004, 23(4):41-45.
[13] 王福荣.生物工程分析与检验.北京:中国轻工业出版社, 2005.141. Wang F R. Biological Engineering Analysis and Inspection.Beijing: China Light Industry Press, 2005.141.
[14] 吴兆亮, 宫立鹏, 赵艳丽, 等.乳链菌肽发酵工艺的研究.化学反应工程与工艺, 2006, 22(4):377-380. Wu Z L, Gong L P, Zhao Y L, et al, Studies on fermentation technology of nisin.Chemical Reaction Engineering and Technology, 2006, 22(4):377-380.
[15] Lv W, Cong W, Cai Z. Improvement of nisin production in pH feed-back controlled, fed-batch culture by Lactococcus lactis.subsp. lactis. Biotechnology Letters, 2004, 26(22): 1713-1716.
[16] 刘亚丽, 贾士儒, 谭之磊, 等. pH 调控和蔗糖流加控制对乳酸链球菌素发酵的影响. 天津科技大学学报, 2008, 23(3): 5-7. Liu Y L, Jia S R, Tan Z L, et al. Effects of pH control and sucrose feeding rates on nisin production. Journal of Tianjin University of Science & Technology, 2008, 23(3): 5-7.
[17] Todorov S D, Dicks L M. Effect of medium components on bacteriocin production by Lactobacillus plantarum strains ST23LD and ST341LD, isolated from spoiled olive brine. Microbiological Research, 2006, 161(2): 102-108.
[18] 吕燕妮, 李平兰, 周伟. 戊糖乳杆菌 31-1 菌株产细菌素发酵条件优化. 微生物学通报, 2005, 32(3): 13-19. Lv Y N, Li P L, Zhou W.Optimizatioin of bacteriocin produced by Lactobacillus pentosus31-1.Journal of Microbiology Bulletin, 2005, 32 (3):13 -19.
[19] 还连栋, 陶勇, 何松, 等. 乳链菌肽高产菌株的选育及其基因定位. 微生物学报, 1995, 35(5): 364-367. Huan L D, Tao Y, He S, et al, Screening of high yield nisin-producing strains and genes location:Journal of Microbiology, 1995, 35 (5): 364-367
[20] Kim W S, Hall R J, Dunn N W. Improving nisin production by increasing nisin immunity/resistance genes in the producer organism Lactococcus lactis. Applied Microbiology and Biotechnology, 1998, 50(4): 429-433.

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