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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2013, Vol. 33 Issue (12): 121-126    
综述     
乳酸克鲁维酵母的发酵优势及在食品酶制剂领域中的应用
王玉海, 张慧斌, 杨慧敏, 孟青青, 王凤寰
北京工商大学食品学院 食品添加剂与配料北京高校工程研究中心 北京市食品风味化学重点实验室 北京 100048
The Fermentation Advantage of Kluyveromyces lactis and Its Application in the Field of Food Enzyme
WANG Yu-hai, ZHANG Hui-bin, YANG Hui-min, MENG Qing-qing, WANG Feng-huan
Beijing Higher Institution Engineering Research Center of Food Additives and Ingredients, Beijing Key Laboratory of Food Flavor Chemistry, School of Food and Chemical Engineering, Beijing Technology and Business University , Beijing 100048, China
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摘要: 乳酸克鲁维酵母是一种能够以乳酸作为其唯一的碳源和能源的酵母,具有营养要求极其简单、生长旺盛、生物量大、生长温度适应范围广、分泌蛋白能力强,不产生内毒素和对人类安全等优点。其作为宿主系统表达食品用酶也已显示出巨大的潜力。从生物学特性、菌种选择、发酵工艺优化、表达效果分析、外源蛋白修饰加工诸多方面阐述了乳酸克鲁维酵母发酵特点,并指出其具有的独特发酵优势。简述了乳酸克鲁维酵母在食品酶制剂领域中的应用。
关键词: 乳酸克鲁维酵母食品酶制剂外源蛋白    
Abstract: Kluyveromyces lactis is the yeast that can use lactic acid as its sole carbon and energy source, with the extremely simple nutritional requirements, vigorous growth, large biomass growth,temperature adaptation to a wide range, good secreting function of protein, does not produce endotoxin, human security and other advantages. It has also shown great potential as a host system to express food enzyme. From biological characteristics, strain selection, fermentation process optimization, analysis of the effect of expression, modification of the heterologous protein these many aspects to elaborate the fermentation characteristics of Kluyveromyces lactis, and pointed out that it has a unique fermentation advantage. Providing a brief overview of Kluyveromyces lactis used in the field of food enzyme.
Key words: Kluyveromyces lactis    Food enzyme    Heterologous protein
收稿日期: 2013-10-08 出版日期: 2013-12-25
ZTFLH:  Q819  
基金资助: 北京市青年英才计划(YETP1450);大学生科研与创业行动计划资助项目
通讯作者: 王凤寰,E-mail:wangfenghuan@th.btbu.edu.cn     E-mail: wangfenghuan@th.btbu.edu.cn
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引用本文:

王玉海, 张慧斌, 杨慧敏, 孟青青, 王凤寰. 乳酸克鲁维酵母的发酵优势及在食品酶制剂领域中的应用[J]. 中国生物工程杂志, 2013, 33(12): 121-126.

WANG Yu-hai, ZHANG Hui-bin, YANG Hui-min, MENG Qing-qing, WANG Feng-huan. The Fermentation Advantage of Kluyveromyces lactis and Its Application in the Field of Food Enzyme. China Biotechnology, 2013, 33(12): 121-126.

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https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I12/121

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