技术与方法 |
|
|
|
|
新型小片段基因表达载体构建方法 |
金磊1,2, 赵文秀2, 马岚2 |
1. 清华大学生命科学学院 北京 100084; 2. 清华大学深圳研究生院生命与健康学部 深圳 518055 |
|
A Novel Method for Constructing Small Fragment Gene of Expression Vector |
JIN Lei1,2, ZHAO Wen-xiu2, MA Lan2 |
1. School of Life Sciences, Tsinghua University, Beijing 100084, China; 2. Division of Life Science & Health, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China |
[1] 何群力,赵国新,苗立群,等. 靶向Livin 基因 siRNA载体的构建和沉默效应观察. 郑州大学学报(医学版),2007,42(4):617-620. He Q L, Zhao G X, Miao L Q, et al. Construction of siRNA vector targeting human Livin gene and observation of its silence efficiency. Journal of Zhengzhou University(Medical Sciences), 2007,42(4): 617-620. [2] Jonathan Silver, Vijaya Keerikatte. Novel use of polymerase chain reaction to amplify cellular DNA adjacent to an integrated provirus. Journal of Virology, 1989,63(5):1924-1928. [3] 陈尚武,马涧泉. 产物克隆方法的新进展.生物技术,1995,5(4):1-4. Chen S W, Ma J Q. The Progress of clone PCR products.Biotechnology,1995,5(4):1-4. [4] 杜广营,朱乃硕. 利用同序异尾限制性内切酶快速克隆多基因片段的新方法.生物化学与生物物理进展,2006,33(6):584-588. Du G Y, Zhu N S. A novel method for quickly cloning genes with multiple DNA fragments using isoschizomer-heterotail restriction endonuclease(IHRE). Prog Biochem Biophys, 2006,33(6):584-588. [5] 赵国新,徐慧丹,赵新河,等. 针对Cox-2基因siRNA载体构建和沉默效应. 河南科技大学学报(医学版),2007,25(1):1-4. Zhao G X, Xu H D, Zhao X H, et al. Construction of a siRNA vector targeting human Cox-2 gene and silence effect. J Henan Univ Sci Tech(Med Sci), 2007,25(1):1-4. |
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|