不同缺氮营养水平对金色奥杜藻生长及光合生理的影响

中国生物工程杂志

2012, Vol. 32

Issue (6): 48-56

研究报告

不同缺氮营养水平对金色奥杜藻生长及光合生理的影响

王璐瑶, 桑敏, 李爱芬, 张成武

暨南大学水生生物研究中心广州 510632

Effects of Different Nitrogen Nutrition Level on the Growth and Photosynthetic Physiology of Odontella aurita

WANG Lu-yao, SANG Min, LI Ai-fen, ZHANG Cheng-wu

Institute of Hydrobiology, Jinan University, Guangzhou 510632, China

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摘要： 采用了Φ6 cm柱状光生物反应器,在不同氮素营养条件(17.6 mmol/L N、8.8 mmol/L N、5.87 mmol/L N、0 mmol/L N)下通气培养硅藻金色奥杜藻,分析探讨藻细胞的光合生理及生长状况与氮素营养水平的关系。结果表明,不同氮素实验组藻细胞达到最大生长的时间明显差异,与对照组(17.6 mmol/L)相比,氮限制(5.87 mmol/L N、8.8 mmol/L N)在培养的前期对金色奥杜藻的生长具有促进作用,氮饥饿(0 mmol/L)显著抑制藻细胞生长(P<0.05)。氮限制实验组藻细胞总碳水化合物的含量显著增加(P<0.05),而总蛋白含量明显下降(P<0.05)。藻细胞叶绿素a、c及总类胡萝卜素含量与培养液的氮素营养水平呈正相关。藻细胞最大光合放氧速率P_m随氮浓度下降而降低,呼吸速率R_d呈现相反趋势,PSⅡ最大光能转化效率(Fv/Fm)、实际光能转化效率(Yield)、潜在活性(Fv/Fo)以及相对电子传递效率(ETR)均随氮素限制而显著下降(P<0.05),说明藻细胞的表观光合生理状况与氮素营养水平直接相关。

关键词： 金色奥杜藻; 氮素营养; 叶绿素荧光参数; 生长

Abstract: In order to analysis the effects of different nitrogen nutrition level on the growth and photosynthetic physiology of Odontella aurita, diatoms were cultured under different nitrogen conditions(17.6 mmol/L N, 8.8mmol/L N, 5.87 mmol/L N, 0 mmol/L N), using 6cm columnar light biological reactor. The results showed that the algae of different nitrogen groups reached the maximum growth at different time. In the early of culturing, nitrogen limitation(5.87mmol/L N, 8.8mmol/L N) promoted the growth of Odontella aurita comparing with the control, while nitrogen starvation significantly inhibited the growth of the algae(P<0.05).The carbohydrate content increased significantly (P<0.05) under nitrogen limitation conditon, while total protein content decreased significantly (P<0.05).The content of chlorophyll a, c, total carotenoids of Odontella aurit are positively related with nitrogen nutrition levels of culture medium. The maximum photosynthetic oxygen release rate P_mdecreased with nitrogen concentration, the respiratory rate of R_dpresented the opposite tendency. The maximum efficiency of light energy conversion of PSⅡ (Fv/Fm), the actual energy conversion efficiency(Yield), potential activity(Fv/Fo) and relative electron transfer efficiency (ETR) decreased significantly with the nitrogen limitation. Illuminating that algal cells of apparent photosynthetic physiological status are directly related to the level of nitrogen nutrition.

Key words: Odontella aurita Nitrate limitation Chlorophyll fluorescence parameters Growth

收稿日期: 2011-12-30 出版日期: 2012-06-25

ZTFLH:

Q945

基金资助: 国家"863"计划(2009AA06440)、国家"973"计划(2011CB2009001)、国家自然科学基金(31170337、41176105)、广东省教育部产学研结合项目 (2010A090200008)、广东高校优秀青年创新人才培养计划(LYM 11025)资助项目

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引用本文:

王璐瑶, 桑敏, 李爱芬, 张成武. 不同缺氮营养水平对金色奥杜藻生长及光合生理的影响[J]. 中国生物工程杂志, 2012, 32(6): 48-56.

WANG Lu-yao, SANG Min, LI Ai-fen, ZHANG Cheng-wu. Effects of Different Nitrogen Nutrition Level on the Growth and Photosynthetic Physiology of Odontella aurita. China Biotechnology, 2012, 32(6): 48-56.

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I6/48

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