沙蜇(<em>Stomolophus meleagris</em>)刺丝囊毒素生物活性的初步分析

中国生物工程杂志

2012, Vol. 32

Issue (6): 43-47

研究报告

沙蜇(Stomolophus meleagris)刺丝囊毒素生物活性的初步分析

李荣锋^1,2, 于华华¹, 邢荣娥¹, 刘松¹, 李鹏程¹

1. 中国科学院海洋研究所青岛 266071;
2. 中国科学院研究生院北京 100039

Biological Activities Analysis of the Nematocyst Venom from Jellyfish Stomolophus meleagris

LI Rong-feng^1,2, YU Hua-hua¹, XING Rong-e¹, LIU Song¹, LI Peng-cheng¹

1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2. Graduate University of the Chinese Academy of Sciences, Beijing 100039, China

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摘要： 从沙蜇触手提取刺丝囊细胞毒素,并对该毒素进行溶血活性、致死活性、SOD活性和抗肿瘤活性的研究。结果显示,沙蜇毒素具有明显的溶血活性,其半溶血率(HU₅₀)约为10.5 μg/ml;该毒素还对草鱼显示出较强的致死活性,半致死量(LD₅₀)为50 μg毒素/g鱼;同时该毒素具有明显的SOD活性和抗肿瘤活性,当毒素浓度为18 μg/ml时其总SOD活性为161 U/mg,而毒素浓度为1 mg/ml时,该毒素对肝癌细胞Bel-7402表现出显著的抑制效果,其抑制率达到54.9%。因此,有必要对沙蜇毒素内的生物活性组分进行深入研究,为沙蜇毒素的开发利用提供依据。

关键词： 沙蜇Stomolophus meleagris; 刺丝囊毒素; 溶血活性; 致死活性; SOD活性; 抗肿瘤活性

Abstract: The nematocyst venom was extracted from the tentacle of jellyfish Stomolophus meleagris. Hemolytic activity, lethal activity, SOD activity and antineoplasmic activity of the nematocyst venom was studied and the results showed that approximately 10.5μg/ml of the venom resulted in 50% hemolysis of the erythrocyte suspension and the half lethal dose (LD₅₀) of the venom was about 50 μg venom/g fish. The total SOD activity was about 161 U/mg when the venom protein concentration was 18μg/ml and the inhibition ratio to the hepatoma Bel-7402 was up to 54.9% when the venom protein concentration was 1mg/ml. Therefore, it is of significance to study the bioactive components from the venom, which is useful for the application of the venom of jellyfish Stomolophus meleagris.

Key words: Stomolophus meleagris Nematocyst venom Hemolytic activity Lethal activity SOD activity Antihepatoma activity

收稿日期: 2011-12-01 出版日期: 2012-06-25

ZTFLH:

Q512

基金资助: 国家自然科学基金(41006095)、十二五科技支撑计划(2011BAE06B04)、青岛市科技计划联合基金(08-1-3-51-jch)、广东省中国科学院全面战略合作项目(2010B080703027)资助项目

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引用本文:

李荣锋, 于华华, 邢荣娥, 刘松, 李鹏程. 沙蜇(Stomolophus meleagris)刺丝囊毒素生物活性的初步分析[J]. 中国生物工程杂志, 2012, 32(6): 43-47.

LI Rong-feng, YU Hua-hua, XING Rong-e, LIU Song, LI Peng-cheng. Biological Activities Analysis of the Nematocyst Venom from Jellyfish Stomolophus meleagris. China Biotechnology, 2012, 32(6): 43-47.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I6/43

[1] 高尚武,洪惠馨,张士美. 中国动物志. 北京:科学出版社,2002.27: 225 - 226. Gao S W, Hong H X, Zhang S M. Fauna Sinica. Beijing:Science Press, 2002.27: 225 - 226.
[2] Dong Z J, Liu D Y, Keesing J K. Jellyfish blooms in China: Dominant species, causes and consequences. Mar Pollut Bull, 2010, 60 (7): 954-963.
[3] Catherine L, Louis F. Downward particulate organic carbon export and jellyfish blooms in southeastern Hudson Bay. J Marine Syst, 2011, 88(3): 446-450.
[4] Jun S, Takaya K, Hideyuki T, et al. Distribution of moon jellyfish Aurelia aurita in relation to summer hypoxia in Hiroshima Bay, Seto Inland Sea. Estuar Coast Shelfs, 2010, 86 (3): 485-490.
[5] Brinkman D, Burnell J. Partial purification of cytolytic venom proteins from the box jellyfish, Chironex fleckeri. Toxicon, 2008, 51(5): 853-863
[6] Yu H H, Xing R E, Liu S, et al. Studies on the hemolytic activity of tentacle extracts of jellyfish Rhopilema esculentum Kishinouye: Application of orthogonal test. Int J Biol Macromol, 2007, 40: 276-280.
[7] Li R F,Yu H H, Feng J H, et al. Two-step purification and in vitro characterization of a hemolysin from the venom of jellyfish Cyanea nozakii Kishinouye. Int J Biol Macromol, 2011, 49(1): 14-19
[8] Nagai H, Takuwa-Kuroda K, Nakao M et al. A novel protein toxin from the deadly box jellyfish (Sea Wasp, Habu-kurage) Chiropsalmus quadrigatus. Biosci Biotech Bioch, 2002, 66 (1):97-102.
[9] Nagai, H, Takuwa Kyoko, Nakao, M et al. Isolation and Characterization of a novel protein toxin from the Hawaiian Box jellyfish (Sea Wasp) Carybdea alata. Biochem Bioph Res Co, 2000, 275(2): 589-594.
[10] Feng J H, Yu H H, Li C P, et al. Isolation and characterization of lethal proteins in nematocyst venom of the jellyfish Cyanea nozakii Kishinouye. Toxicon, 2010, 55 (3): 118-125.
[11] Ramasamy S, Isbister G K, Seymour J E, et al. The in vivo cardiovascular effects of the Irukandji jellyfish (Carukia barnesi) nematocyst venom and a tentacle extract in rats. Toxicol Lett, 2005, 155 (1): 135-141.
[12] Ramasamy S, Isbister G K, Seymour J E, et al. Pharmacologically distinct cardiovascular effects of box jellyfish (Chironex fleckeri) venom and a tentacle-only extract in rats. Toxicol Lett, 2005, 155 (2): 219-226.
[13] 李翠萍,于华华,陈晓琳,等. 白色霞水母蛋白抗氧化活性的初步研究. 海洋科学,2008,32(7):65-70. Li C P, Yu H H, Chen X L, et al. Study on the antioxidant activity of the proteins from the jellyfish Cyanea nozakii Kishinouye. Marine Sciences, 2008,32 (7): 65-70.
[14] Li C P, Yu H H, Liu S, et al. Factors affecting the protease activity of venom from jellyfish Rhopilema esculentum Kishinouye. Bioorg Med Chem Lett, 2005, 15 (24): 5370-5374.
[15] Bloom D A, Burnett J W, Alderslade P. Partial purification of box jellyfish (Chironex fleckeri) nematocyst venom isolated at the beachside. Toxicon, 1998, 36 (8): 1075-1085.
[16] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248-254.
[17] Laemmli U K, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227: 680-685.
[18] Switzer R C, Merril C R, Shifrin S. A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem,1979, 98(1):231-237.
[19] Rottini G, Gusani L, Parovel E, et al. Purification and properties of a cytolytic toxin in venom of the jellyfish Carybdea marsupialis. Toxicon, 1995, 33 (3): 315-326.
[20] Yu H H, Li C P, Li R G, et al. Factors influencing hemolytic activity of venom from the jellyfish Rhopilema esculentum Kishinouye, Food Chem Toxicol, 2007, 45 (7):1173-1178.
[21] Feng J H, Yu H H, Xing R E, et al. Partial characterization of the hemolytic activity of the nematocyst venom from the jellyfish Cyanea nozakii Kishinouye. Toxicol in Vitro, 2010, 24 (6): 1750-1756.
[22] Helmholza H, Ruhnaua C, Schuttb C, et al. Comparative study on the cell toxicity and enzymatic activity of two northern scyphozoan species Cyanea capillata (L.) and Cyanea lamarckii (Pe'ron & Le' slieur). Toxicon, 2007, 50 (1): 53-64.
[23] Marino A, Morabito R, Pizzata T, et al. Effect of various factors on Pelagia noctiluca (Cnidaria, Scyphozoa) crude venom-induced haemolysis. Comp Biochem Phys A, 2008,151 (1):418-422.
[24] Yu H H, Liu X G, Xing R G, et al. Radical scavenging activity of protein from tentacles of jellyfish Rhopilema esculentum. Bioorg Med Chem Lett, 2005, 15(10): 2659-2664.

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