褪黑素对绒山羊皮肤FGF5等绒毛生长相关基因的影响

中国生物工程杂志

2012, Vol. 32

Issue (6): 20-26

研究报告

褪黑素对绒山羊皮肤FGF5等绒毛生长相关基因的影响

赵德超, 付绍印, 吕晓曼, 吴江鸿, 张燕军, 李金泉, 王志新, 张永斌, 张文广

内蒙古农业大学动物科学学院动物遗传育种与繁殖自治区重点实验室呼和浩特 010018

Effect of Melatonin on Genes Including FGF5 That Related to Hair Growth in Cashmere Goat Skin

ZHAO De-chao, FU Shao-yin, LV Xiao-man, WU Jiang-hong, ZHANG Yan-jun, LI Jin-quan, WANG Zhi-xin, ZHANG Yong-bin, ZHANG Wen-guang

China, College, of, Animal, Science, Key, Laboratory, of, Animal, Genetics, Breeding, and, Reproduction, Inner, Mongolia, Autonomous, Region, Inner, Mongolia, Agricultural, University, Huhhot, 010018, China

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摘要： 目的:旨在分析埋植褪黑素后对绒山羊皮肤绒毛生长相关基因表达的影响,进而探讨褪黑素促绒毛生长的机理。方法:以内蒙古白绒山羊为研究对象,选取体况相近的4月龄母羔羊10只,按试验要求随机分为2组,处理组按照2mg/kg BW(体重)的剂量每两个月耳后皮下埋植褪黑素,对照组不埋植,试验时间为1年。实验开始后每月采集绒山羊体侧皮肤和毛样,利用荧光定量PCR技术检测9个绒毛生长相关基因的表达变化。结果:(1)埋植褪黑素后各个基因的平均表达水平都有所上调,其中显著上调FGF5(P<0.0001),而对β-catenin、MSX-2、 Wnt10b、 BMP2、BMPRIB、NTRK3、Delta1、hairless等无显著影响(P>0.05);(2)埋植褪黑素明显提高了FGF5与hairless的相关性,并且使FGF5与β-catenin、NTRK3的相关性由负相关变成正相关,与Wnt10b的相关性降低,FGF5与其余4个基因的相关性变化不大。结论:结果提示,褪黑素上调绒山羊皮肤FGF的表达,影响FGF5与hairless、β-catenin、NTRK3之间的相关性;山羊中FGF5基因对被毛表型的作用与其他动物不同;褪黑素对复杂组织和单个细胞的作用不同;MAPK信号通路是受褪黑素影响最大的一个信号途径。推测褪黑素可能通过调节绒山羊皮肤中RORα来发挥作用,影响FGF5与hairless、β-catenin、NTRK3和Wnt10b等的相关性,同时抑制MSX2和BMP2的功能,进而促进绒毛的生长。

关键词： 褪黑素; 绒山羊; FGF5; 绒毛生长机理

Abstract: The aim is to study the effect of melatonin on genes which related to hair growth on skin, and explore the mechanism of melatonin in cashmere growth. Using the Inner Mongolia white cashmere goats as the research object, 10 lambs on 4 months age with similar body condition were selected, and divided into 2 groups randomly. Following testing requirements, the treatment group was implanted melatonin in subcutaneous behind the ear every two months according to 2mg / kg BW (body weight) dose, and nothing to the control. The experiment time was last for one year. Then the cashmere goat skin and hair in the body side were gathered, using real-time PCR to detect the expression changes of 9 genes related to hair growth. The results showed that (1)melatonin implanting can up-regulate FGF5’s expression significantly (P<0.0001), but has no significantly effect to β-catenin, MSX-2, Wnt10b, BMP2, BMPRⅠB, NTRK3, Delta1and hairless (P>0.05); (2) melatonin implanting obviously improved the correlation between FGF5 and hairless, and make the negative correlation turn to positive correlation on β-catenin and NTRK3, the correlation with Wnt10b was reduced, while the other 4 were little changed. These results indicated that Melatonin up-regulate the expression of FGF5 in cashmere goat skin, impact the correlation of FGF5 with hairless, β-catenin and NTRK3; the impact of melatonin on hair is different with other animals; the effect of melatonin on complex organizational and individual cells are different. MAPK signaling pathway is the greatest impacted by melatonin. It is putative that melatonin can regulate RORα in cashmere goat skin to impact the correlation of FGF5 with hairless, β-catenin, NTRK3 and Wnt10b, and inhibit the function of MSX2 and BMP2 for promoting hair growth.

Key words: Melatonin FGF5 Cashmere goat Mechanism of hair growth

收稿日期: 2011-12-07 出版日期: 2012-06-25

ZTFLH:

Q819

基金资助: 国家科技支撑项目(2011BAD28B05)、国家自然科学基金(30960246)资助项目

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引用本文:

赵德超, 付绍印, 吕晓曼, 吴江鸿, 张燕军, 李金泉, 王志新, 张永斌, 张文广. 褪黑素对绒山羊皮肤FGF5等绒毛生长相关基因的影响[J]. 中国生物工程杂志, 2012, 32(6): 20-26.

ZHAO De-chao, FU Shao-yin, LV Xiao-man, WU Jiang-hong, ZHANG Yan-jun, LI Jin-quan, WANG Zhi-xin, ZHANG Yong-bin, ZHANG Wen-guang. Effect of Melatonin on Genes Including FGF5 That Related to Hair Growth in Cashmere Goat Skin. China Biotechnology, 2012, 32(6): 20-26.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I6/20

[1] 李金泉,尹俊,周欢敏. 绒山羊生长调控机理研究进展. 中国草食动物,2001,03-0041-04. Lin J Q, Yin J, Zhou H M. The advance of research on mechanism of the regulation of Cashmere growth. China herbivores, 2001,03-0041-04.
[2] 贾志海,蔡青和. 山羊绒生长机制及其营养研究进展. 动物营养学报,1999:12(11):97-102. Jia Z H, Cai Q H. The advance of research on growth mechanism and nutrient requirement of Cashmere. Acta Zoonutrimenta Sinica, 1999,12(11):97-102.
[3] Ma X, Idle J R, Krausz K W, et al. Metabolism of melatonin by human cytochromes p450. Drug Metabolism and Disposition, 2005,33 (4):489.
[4] Slominski A, Pisarchik A, Semak I, et al. Serotoninergic and melatoninergic systems are fully expressed in human skin. Faseb Journal, 2002,16 (6):896-898.
[5] Slominski A T, Pisarchik A, Zbytek B, et al. Serotoninergic and melatoninergic systems expressed in the skin. Journal of Investigative Dermatology, 2003,96(1):143-153.
[6] Kobayashi H, Kromminga A, Dunlop T W, et al. A role of melatonin in neuroectodermal-mesodermal interactions: the hair follicle synthesizes melatonin and expresses functional melatonin receptors. Faseb Journal, 2005,19 (9):1710-1712.
[7] Fischer T W, Slominski A, Tobin D J, et al. Melatonin and the hair follicle. J Pineal Res, 2008,44 (1):1-15.
[8] 孔庆松,刘志平,景松岩. 褪黑素促进水貂冬皮早熟的研究. 野生动物,1995,4:22-26. Kong Q S, Liu Z P, Jing S Y, et al. Study on advancing the maturation of winter fur in mink treated with melatonin. Chinese Wildlife,1995, 4:22-26.
[9] 王孝胜,张志明,毕全秀. 埋植褪黑素促进水貂生长、换毛、毛皮早熟的试验报告.毛皮动物饲养,1995,(2):3-5. Wang X S, Zhang Z M, Bi Q X. The experiment report of melatonin implantation promote mink growth, molting, fur precocious. Journal of Economic Animal, 1995, 2: 3-5.
[10] 柳建昌,尹协镇,方天祺. 褪黑素对中国绒山羊在非生绒期促绒生长与绒产量的影响.动物学杂志,1998,33(3):8-12. Liu J C, Yin X Z, Fang T Q. Effect of melatonin on the cashmere growth and production of Chinese White Goats. Chinese Journal of Zoology, 1998,33(3):8-12.
[11] Nixon A J, Choy V J, Parry A L, et al. Fiber growth initiation in hair follicles of goats treated with melatonin. Journal of Experimental Zoology, 2005,267 (1):47-56.
[12] Betteridge K,Devantier B P,Welch R A,et al. Out of season cashmere growth in feral goats. New Zealand: Lincoln college Press. In Proceedings of the 2nd International Cashmere Congress,1987,137-144.
[13] Welch R A,Gurnsey M P,Betteridge K,et al. Goat fibre response to melatonin given in spring in two consecutive years. Proc NZ Soc Anim Prod, 1990,50:335-358.
[14] Kloren W R,Nprton B W, Waters M J. Fleece growth in Australian cashmere goats. The effects of nutrition and age on fleece growth, prolactin and thyroxine concentration, Australian Journal of Agricultural Research, 1993,44(5):1003-1021.
[15] 贾志海,安民. 山羊绒生长机理研究进展. 草食家畜,1996,1:2-6. Jia Z H, An M. The advance of research on mechanism of Cashmere growth. Herbivorous Animals, 1996,1: 2-6.
[16] 常子丽.皮肤毛囊基因调控网络的初建及其在绒山羊上的功能预测. 呼和浩特:内蒙古农业大学,2007. Chang Z L. Primary construction for gene regulatory network of the skin follicle and functional prediction in Cashmere Goats. Hohhot:Inner Mongolia Agricultural University, 2007.
[17] 岳春旺,张微,孔祥浩,等. 褪黑素对内蒙古白绒山羊产绒性能的影响. 中国畜牧杂志,2007,47 (3):32-34. Yue C W, Zhang W, Kong X H, et al. Effect of melatonin on Cashmere performance in Inner Mongolia white cashmere goats. Chinese Journal of Animal Science, 2007, 47 (3): 32-34.
[18] Hebert J M,Rosenquist T,Gotz J,et al. FGF5 as a regulator of the hair growth cycle:evidence from targeted and spontaneous mutations.Cell,1994,78(6):1017-1025.
[19] Sundberg J P,Rourk M H,Boggess D, et al. Angora mouse mutation:altered hair cycle,follicular dystrophy,phenotypic maintenance of skin grafts,and changes in keratin expression.Veterinary Pathology,1997,34(3):171-179.
[20] Housley D J,Venta P J. The long and the short of it:evidence that FGF5 is a major determinant of canine 'hair’-itability. Animal Genetics,2006,37(4):309-315.
[21] Rougeot J. The bault RG.Seasonal-variations in pelage composition and structure with special reference to angorarabbit. Annales De Zootechnie,1983,32(3):287-314.
[22] 刘海英,杨桂芹,张微等. FGF5基因对内蒙古绒山羊绒毛性状的影响. 遗传,2009,31(2):175-179. Liu H Y, Yang G Q, Zhang W, et al. Effects of FGF5 gene on fibre traits on Inner Mongolian cashmere goats. Hereditas, 2009, 31(2):175-179.
[23] Tsuji H, Ishida-Yamamoto A, Takahashi H,et al. Nuclear localization of beta-catenin in the hair matrix cells and differentiated keratinocytes. J Dermatol Sci, 2001,27 (3):170-177.
[24] Ridanpaa M, Fodde R, Kielman M. Dynamic expression and nuclear accumulation of beta-catenin during the development of hair follicle-derived structures. Mech Dev, 2001,109 (2):173-181.
[25] Jamora C, Lee P, Kocieniewski P,et al. A signaling pathway involving TGF-beta2 and snail in hair follicle morphogenesis. PLoS Biol, 2005,3 (1):e11.
[26] Zarach J M, Beaudoin G M,Coulombe P A,et al. The co-repressor hairless has a role in epithelial cell differentiation in the skin. Development, 2004,131 (17):4189.
[27] Jiang T X, Liu Y H, Widelitz R B,et al. Epidermal dysplasia and abnormal hair follicles in transgenic mice overexpressing homeobox gene MSX-2. Invest Dermatol, 1999,113 (2):230-237.
[28] Wang W P, Widelitz R B, Jiang T X,et al. Msx-2 and the regulation of organsize: epidermal thickness and hair length. J Investig Dermatol Symp Proc, 1999,4 (3):278-281.
[29] Stenn K S, Nixon A J, Jahoda C A,et al. What controls hair follicle cycling?. Exp Dermatol, 1999,8(4): 229-236.
[30] Huang J, Dattilo L K, Rajagopal R,et al. FGF-regulated BMP signaling is required for eyelid closure and to specify conjunctival epithelial cell fate. Development, 2009,136 (10):1741-1750.
[31] Xu R H. FGF, BMP, and TGFβ signaling together control human embryonic stem cell fates. Cell Research, 2008,18(29):119.
[32] Aranda A, Pascual A. Nuclear hormone receptors and gene expression. Physiological Reviews, 2001,81 (3):1269-1304.
[33] Wiesenberg I,Missbach M, Kahlen J P,et al. Transcriptional activation of the nuclear receptor RZR {alpha} by the pineal gland hormone melatonin and identification of CGP 52608 as a synthetic ligand. Nucleic Acids Research, 1995,23 (3):327.
[34] Shibata H, Spencer T E, Ońate S A,et al. Role of co-activators and co-repressors in the mechanism of steroid/thyroid receptor action. Recent Progress in Hormone Research, 1997,52:141-164.
[35] Fischer T W, Slominski A, Tobin D J,et al. Melatonin and the hair follicle. Journal of Pineal Research, 2008,44(1):1-15.

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