三叶半夏(<em>Pinelliaternata</em> (Thunb.) Breit)的间歇浸没培养

中国生物工程杂志

2012, Vol. 32

Issue (11): 49-54

研究报告

三叶半夏(Pinelliaternata (Thunb.) Breit)的间歇浸没培养

贾明良^1,2, 张本厚², 高伟平², 陈集双^1,2, 欧阳平凯¹

1. 南京工业大学生物资源工程研究所南京 210009;
2. 南京工业大学大丰海洋产业研究院大丰 224145

Micropropagation of Pinellia Ternata (Thunb.) Breit in a Bioreactor Using Temporary Immersion System

JIA Ming-liang^1,2, ZHANG Ben-hou², GAO Wei-ping², CHEN Ji-shuang^1,2, OUYANG Ping-kai¹

1. Institute of Bioresourse Engineering, Nanjing University of Technology, Nanjing 210009, China;
2. Dafeng Marine Industrial Institute, Nanjing University of Technology, Dafeng 224145, China

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摘要： 目的:利用间歇浸没培养反应器对药用植物三叶半夏(Pinellia ternata (Thunb.) Breit)进行组培快繁研究。方法:以增殖倍数、鲜重、平均鲜重、折干率和组培苗状态为指标,一方面对三叶半夏叶片、叶柄及丛生芽体进行培养,考察适合间歇浸没培养的外植体类型;另一方面以叶柄为外植体对三叶半夏进行间歇浸没培养、固体培养和液体摇瓶培养,考察三种培养方式对其生长的影响。结果:不同的外植体在反应器内培养时,从增殖情况来看,丛生芽作为外植体得到的增殖倍数高达39.15,叶柄为27.65,叶片为18.05。鲜重方面与增殖倍数具有相同的规律。从平均鲜重和折干率来看,无显著差异。由组培苗形态来看,以叶柄诱导的组培苗最为健壮,而以丛生芽诱导的组培苗则较为细弱。不同培养方式下的增殖倍数分别为:间歇浸没培养方式最高为24.73,其次固体培养为14.75,液体摇瓶培养为12.26,差异显著。平均鲜重和折干率均为间歇浸没培养最优。由组培苗形态来看固体培养优于间歇浸没培养,反应器得到的组培苗较为细弱。在保卫细胞长宽和叶绿体个数上两者没有显著性差异。而液体摇瓶培养玻璃化导致叶绿体个数减少,不适合培养三叶半夏的。结论:较适合间歇浸没培养的外植体类型为叶柄和丛生芽,以叶柄为优。间歇浸没培养方式相对于传统的固体和液体培养在各项生理指标上表现较好,具有大规模培养三叶半夏的潜力。

关键词： 三叶半夏; 生物反应器; 间歇浸没培养; 植物组织培养

Abstract: Objactive: To improve a new system for micropropagation of Pinellia ternata (Thunb.) Breit. in a temporary immersion bioreactor. Methods: Average number of tubers per plantlet (NTPs), Fresh weight (FW), Average fresh weight per plantlet (FWPs), Dry weight/Fresh weight (DW/FW) and the status of plantlet were studied refering standards. Firstly, leaves, petioles and cluster buds were cultured to establish the best explants type in the temporary immersion system. Secondly, petioles were cultured in the temporary immersion system, solid and liquid culture methods to compare the training effect. Results: Different types of explants were cultured in this temporary immersion system. Highest NTPs of 39.15 was achieved by culturing cluster bud. The petiole was 27.65 and the leaf was 18.05. Fresh weight has the same rule with NTPs. FWPs and DW/FW both have no significant differences. The plantlets induced by petioles were the most robust and which were more in thin induced by cluster buds. NTPs induced by petioles were achieved by different culture methods were as follows: temporary immersion system was 24.73, solid culture method was 14.75 and liquid culture method was 12.26. FWPs and DW/FW of temporary immersion system were optimal. The solid method and temporary immersion system were the suitable culturing methods for. The status of plantlets cultured by solid method was better than temporary immersion system. And the plantlets of temporary immersion system were thinner. Length of stoma guard cell (LSGC), Width of stoma guard cell (WSGC) and Average number of chloroplast per stoma guard cell (NCPs) have no significant difference between the two methods. The vitrification of plantlets cultured by liquid method has less number of chloroplasts, so the liquid method was not suitable for cultivation of Pinellia ternata (Thunb.) Breit. Conclusion: Petioles and cluster buds are suitable for the temporary immersion culture, and the petiole is better. Compared with conventional solid and liquid culture, temporary immersion culture system behaved as the best in various physiological indexes and that this system has the potential of large-scale micropropagation for this medicinal plant.

Key words: Pinellia ternata (Thunb.) Breit Bioreactor Temporary immersion system Plant tissue culture

收稿日期: 2012-09-19 出版日期: 2012-11-25

ZTFLH:

Q819

基金资助: 江苏省高层次创新创业人才引进计划资助项目(51207204)

通讯作者: 陈集双,电子信箱:biochenjs@njut.edu.cn E-mail: biochenjs@njut.edu.cn

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引用本文:

贾明良, 张本厚, 高伟平, 陈集双, 欧阳平凯. 三叶半夏(Pinelliaternata (Thunb.) Breit)的间歇浸没培养[J]. 中国生物工程杂志, 2012, 32(11): 49-54.

JIA Ming-liang, ZHANG Ben-hou, GAO Wei-ping, CHEN Ji-shuang, OUYANG Ping-kai. Micropropagation of Pinellia Ternata (Thunb.) Breit in a Bioreactor Using Temporary Immersion System. China Biotechnology, 2012, 32(11): 49-54.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I11/49

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