转基因抗虫棉棉籽组分的代谢组学研究

中国生物工程杂志

2012, Vol. 32

Issue (11): 35-41

研究报告

转基因抗虫棉棉籽组分的代谢组学研究

孙彩霞¹, 汪莹¹, 吴晓菲¹, 陈利军², 武志杰²

1. 东北大学生物技术研究所沈阳 110819;
2. 中国科学院沈阳应用生态研究所沈阳 110016

Metabonomic Study on the Composition of Insect-resistant Transgenic Cottonseeds

SUN Cai-xia¹, WANG Ying¹, WU Xiao-fei¹, CHEN Li-jun², WU Zhi-jie²

1. Institute of Biotechnology, Northeastern University, Shenyang 110819, China;
2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China

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摘要： 采用核磁共振氢谱(¹H NMR)获取不同转基因抗虫棉棉籽及其非转基因对照棉籽的代谢物谱,并结合多维统计分析探查植物基因工程操作是否引发棉籽中代谢产物种类和数量的非预期改变。结果表明,不同棉籽的¹H NMR谱图轮廓基本特征相似,均可分为氨基酸区、碳水化合物区及芳香区;当谱图按分区扩展后,多处峰信号在转基因与非转基因对照间有明显的不同。采用偏最小二乘法-判别分析(PLS-DA)可实现转基因抗虫棉棉籽与非转基因对照的代谢轮廓判别且分类的效果优于主成分分析(PCA)方法。转基因抗虫棉籽与其非转基因对照间的主要差异代谢物与初级氮代谢、三羧酸循环及脂肪酸代谢有关。

关键词： ¹H; NMR; 转基因抗虫棉棉籽; 代谢组学; 多维统计分析; 安全性评价

Abstract: The term of metabolome has been used to describe the responses of plant to external perturbations recently. The study of metabolite profiling of different insect-resistant cottonseeds and their non-transgenic counterparts using ¹H NMR and multivariate analysis technique was performed to investigate the unintended metabolic variations associated with genetic modifications. The results obtained showed that the overall appearance of the spectrum was quite similar among different cottonseeds. However, there were many different peak signals in the animo acids region (3～0.5 ppm) of the expansion spectra of transgenic cottonseeds when compared with their controls. Although score plots generated using principal component analysis showed the potential to distinguish transgenic cottonseeds from non-transgenic controls, a better classification between them was obtained by partial least square-discriminant analysis. The major compounds contributing to the discrimination were those metabolites that involved the metabolic pathway of fatty acid, the primary nitrogen metabolism and the tricarboxylic acid cycle.

Key words: ¹H NMR Insect-resistant transgenic cottonseeds Metabonomics Multivariate analysis Safety assessment

收稿日期: 2012-06-21 出版日期: 2012-11-25

ZTFLH:

O657.3

基金资助: 中央高校基本科研业务费专项基金(N090405011);中国科学院知识创新工程项目(KZCX2-EW-413);教育部留学回国人员科研启动基金资助项目

通讯作者: 孙彩霞,电子信箱:suncaixia@hotmail.com E-mail: suncaixia@hotmail.com

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引用本文:

孙彩霞, 汪莹, 吴晓菲, 陈利军, 武志杰. 转基因抗虫棉棉籽组分的代谢组学研究[J]. 中国生物工程杂志, 2012, 32(11): 35-41.

SUN Cai-xia, WANG Ying, WU Xiao-fei, CHEN Li-jun, WU Zhi-jie. Metabonomic Study on the Composition of Insect-resistant Transgenic Cottonseeds. China Biotechnology, 2012, 32(11): 35-41.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I11/35

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