PES1与雌激素受体存在相互作用

中国生物工程杂志

2012, Vol. 32

Issue (08): 14-18

研究报告

PES1与雌激素受体存在相互作用

李杰萍¹, 庄庆仁¹, 兰小鹏², 曾国彬¹, 罗小锋¹

1. 武警福建总队医院福州 350003;
2. 南京军区福州总医院检验医学研究所福州 350003

PES1 Interacts with Estrogen Receptor

LI Jie-ping¹, ZHUANG Qin-ren¹, LAN Xiao-peng², ZENG Guo-bin¹, LUO Xiao-feng ¹

1. Department of Clinical Medical Laboratory, General Hospital of Fujian Corps of CAPF, Fuzhou 350003, China;
2. Institute of Clinic Lab Medicine, Fuzhou General Hospital of Nanjing Military Command, PLA, Fuzhou 350003, China

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摘要： 雌激素(E₂)和雌激素受体(ER)在E₂诱发的肿瘤中起着极其重要的作用。ER共调节因子通过与ER相互作用调节其生物学功能。PES1主要表达于E₂的重要靶器官如乳腺、卵巢等组织中,并在乳腺癌细胞中高表达。用PCR技术构建HA标签的PES1全长以及1~322aa、312~588aa和414~588aa三个不同功能区片段的重组质粒。将不同的重组质粒与FLAG-ERα和或FLAGC-ERβ共转染293T细胞后进行免疫共沉淀,以验证PES1与ER是否有相互作用以及相互作用的区域。用含雌激素受体作用元件的荧光素酶报告基因(ERE-LUC)检测PES1对ERα和ERβ转录激活活性的影响。结果表明,PES1与ERα和ERβ均相互作用,且PES1的1~322aa区域与ERα和ERβ相结合。PES1能特异地、E₂非依赖性抑制ERβ的转录激活活性。实验结果显示,PES1是一个新的ER共调节因子,需要进一步研究其在ERβ信号通路及其在E₂诱发的肿瘤的作用。

关键词： PES1; 雌激素受体; 共调节因子

Abstract: Estrogen(E₂) and estrogen receptors (ER) play a vital role in the E₂-induced neoplasms. The coregulators of ERs modulate their biological functions by binding these receptor. PES1, as a potential regulator, is mainly expressed in the target organs such as breast and ovarian, and also, is highly expressed in the breast cancer cells. In current work, the HA-tagged recombinant plasmids of full-length PES1, and its different function regions(1~322aa, 312~588aa and 414~588aa) were constructed by PCR. To test whether PES1 can interact with ERs and their interaction regions, different recombinant plasmids constructed were co-transfected with FLAG-ERα or/and FLAGC-ERβ in 293T cells before co-immunoPrecipitation (co-IP). The co-IP results showed that PES1 could interact with ERα and ERβ by binding their 1~322aa region. Further experiments demonstrated that PES1 specifically inhibited the transactivation activity of ERβ in E₂-independent manner by analyzing estrogen receptor element luciferase (ERE-LUC). These results suggested that PES1 may act as a new ER coregulator. Further mechanisms and roles in the ERβ signaling pathway and E₂-induced neoplasms remain to be determined.

Key words: PES1 Estrogen receptor Co-regulator

收稿日期: 2012-04-09 出版日期: 2012-08-25

ZTFLH:

Q789

基金资助: 福建省社会发展科技重点项目(2010Y0048)和福建省自然科学基金(2010J05082)资助项目

通讯作者: 李杰萍 E-mail: liejeping@hotmail.com

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引用本文:

李杰萍, 庄庆仁, 兰小鹏, 曾国彬, 罗小锋. PES1与雌激素受体存在相互作用[J]. 中国生物工程杂志, 2012, 32(08): 14-18.

LI Jie-ping, ZHUANG Qin-ren, LAN Xiao-peng, ZENG Guo-bin, LUO Xiao-feng. PES1 Interacts with Estrogen Receptor. China Biotechnology, 2012, 32(08): 14-18.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2012/V32/I08/14

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