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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2011, Vol. 31 Issue (5): 75-80    
研究报告     
渗透压在葡萄酒酵母代谢中的调控作用
谢涛, 张儒
湖南工程学院化学化工学院 湘潭 411104
Osmotic Press Plays an Important Role in Metabolic Control of Saccharomyces ellipsoideus
XIE Tao, ZHANG Ru
Department of Chemical Engineering, Hunan Institute of Engineering, Xiangtan 411104, China
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摘要：

以磷酸丙糖异构酶部分缺失突变株做对比,研究了渗透压对葡萄酒酵母发酵过程中甘油合成与挥发酸生成的调节作用。结果表明:渗透压对野生型葡萄酒酵母中存在的磷酸二羟丙酮(DHAP)与3-磷酸甘油醛(GA3P)平衡具有调节作用,能使平衡向磷酸二羟丙酮方向迁移以合成更多的甘油,而当磷酸丙糖异构酶部分缺失时渗透压对这一平衡基本不起作用;在相同条件下,磷酸丙糖异构酶部分缺失突变株较之葡萄酒酵母野生株的甘油合成量大大减少,其胞内主要积累3-磷酸甘油醛从而更多地生成酵解代谢产物。因此,在适当高渗环境中,葡萄酒酵母野生株胞内积累磷酸二羟丙酮有利于甘油与挥发酸比例的调控。
关键词： 渗透压;  葡萄酒酵母;  代谢调控;  磷酸丙糖异构酶    
Abstract:

Using triose phosphate isomerase deficient (tpiΔ) mutant of Saccharomyces ellipsoideus as the control, the regulation of osmotic press on the balance between glycerol production and volatile acid formation was first studied. The results demonstrated that there existed the control of osmotolerance on the balance between dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) in wild S. ellipsoideus and the suitable high osmotolerance can transport metabolic flux from GA3P to DHAP to produce much glycerol. However, the control of osmotolerance didn't exist in tpiΔ mutant of S. ellipsoideus. In comparison with wild S. ellipsoideus, the tpiΔ mutant greatly reduced the yield of glycerol production and much created glycolytic metabolites because of GA3P accumulation at the same conditions. Therefore, the conclusion was made that, at the suitable high osmotolerance, DHAP accumulation of wild S. ellipsoideus would be helpful to regulate the ratio of glycerol to volatile acid.
Key words: Osmotolerance    Saccharomyces ellipsoideus    Metabolic regulation    Triosephosphate isomerise(TPI)
收稿日期: 2010-10-19 出版日期: 2011-05-27
ZTFLH:  TQ92  
基金资助:

湖南省自然科学基金资助项目(07JJ6031)
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引用本文:

谢涛, 张儒. 渗透压在葡萄酒酵母代谢中的调控作用[J]. 中国生物工程杂志, 2011, 31(5): 75-80.

XIE Tao, ZHANG Ru. Osmotic Press Plays an Important Role in Metabolic Control of Saccharomyces ellipsoideus. China Biotechnology, 2011, 31(5): 75-80.

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https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2011/V31/I5/75


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