技术与方法 |
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利用Red重组系统快速构建基因打靶载体 |
姜丽1, 叶湘漓2, 李大力1 |
1. 华东师范大学生命科学学院生命医学研究所 上海 200241;
2. 湖南师范大学医学院 长沙 410013 |
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Construction of Mouse Gene-targeting Vector Through Modified Recombineering Strategy |
JIANG Li1, YE Xiang-li2, LI Da-li1 |
1. Institute of Biomedical Sciences, East China Normal University, Shanghai 200241, China;
2. College of Medicine, Hunan Normal University, Changsha 410013, China |
[1] 杨建岭,顾淑萍,陈臣,等. 用Red/ET重组酶构建基因打靶载体. 生物工程学报, 2006, 22(6)::919-924. Yang J L, Gu S P, Chen C, et al. Chinese J of Biotechnology, 2006, 22(6):919-924.
[2] 李湘萍,徐慰倬,李宁. 动物基因敲除研究的现状与展望. 遗传, 2003, 25(1):81-88. Li X P, Xu W Z, Li N. Hereditary, 2003, 25(1):81-88.
[3] Wang B, Zhou J F. Specific genetic modifications of domestic animals by gene targeting and animal cloning. Reprod Biol Endocrinol, 2003, 1(103):1-8.
[4] Wu S, Ying G X, Wu Q, et al. A protocol for constructing gene targeting vectors: generating knockout mice for the cadherin family and beyond. Nat Protoc, 2008, 3(6):1056-1076.
[5] 王军平,张友明. Red/ET重组在基因打靶载体快速构建中的应用. 遗传, 2005, 27(6): 953-958. Wang J P, Zhang Y M. Yi Chuan, 2005, 27(6):953-958.
[6] Zhang Y M, Buchholz F, Muyrers J P, et al. A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet, 1998, 20(2):123-128.
[7] Zhang Y M, Muyrers J P, Testa G, et al. DNA cloning by homologous recombination in Escherichia coli. Nat Biotechnol, 2000, 18(12):1314-1317.
[8] Valenzuela D M, Murphy A J, Frendewey D, et al. High-throughput engineering of the mouse genome coupled with high-resolution expression analysis. Nat Biotechnol, 2003, 21(6):652-659.
[9] Liu P T, Jenkins N A, Copeland N G. A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res, 2003, 13(3):476-484.
[10] Lee E C, Yu D G, Velasco J M, et al. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics, 2001, 73(1):56-65.
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