表达乙肝病毒受体人ASGPR转基因小鼠的建立

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中国生物工程杂志 ›› 2010, Vol. 30 ›› Issue (05) : 87-91.

技术与方法

表达乙肝病毒受体人ASGPR转基因小鼠的建立

郭虹敏^1,2,吴晓洁²,李想³,周艳荣²,林艳丽²,熊福银²,薛世伟¹,陈红星^2**陈树林^1**

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Establishment of the Transgenic Mice Expressing the HBV Receptor Human ASGPR

GUO Hong-min^1,2,WU Xiao-Jie²,LI Xiang³,ZHOU Yan-rong²,LIN Yan-li²,XIONG Fu-yin²,XUE Shi-wei¹,CHEN Hong-xing²,CHEN Shu-lin¹

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摘要

目的：建立表达乙肝病毒受体人ASGPR的转基因小鼠。方法：克隆人的脱唾液酸糖蛋白受体（ASGPR）两个亚基的cDNA，连入PCAGGS构建转基因表达载体，以显微共注射的方法将两种各3.9kb的转基因片段引入小鼠的受精卵。采用PCR、Southern印迹、RT-PCR、Western印迹的方法对转基因小鼠进行鉴定。结果与结论：获得了在小鼠肝脏组织中共表达有乙肝病毒（HBV）受体ASGPR H1和ASGPR H2的一个转基因小鼠系，可为HBV的研究提供一种良好的感染动物模型。

Abstract

Objective:To generate the transgenic mice that express human Asialoglycoprotein receptor(ASGPR),which is considered as one of the hepatitis B virus (HBV) receptors. Methods: Both cDNA of the Human ASGPR H1&H2 subunits were cloned and the expression vector PCAGGS-ASGPRH1&H2 were constructed, Both of the 3.9kb DNA fragments were introduced into the fertilized eggs of mice by microinjection. The genotypes of the transgenic mice were identified by PCR and Southern-blot methods, and the expression of the exogenous ASGPR gene was confirmed by the RT-PCR and Western blot. Result and Conclusion: One transgenic mouse line that express the human ASGPR was obtained. It can be used as an animal model for the research of HBV infection.

导出引用

郭虹敏吴晓洁李想周艳荣林艳丽熊福银薛世伟陈红星陈树林. 表达乙肝病毒受体人ASGPR转基因小鼠的建立[J]. 中国生物工程杂志, 2010, 30(05): 87-91

GUO Gong-Min, TUN Xiao-Ji, LI Xiang, ZHOU Yan-Rong, LIN Yan-Li, XIONG Fu-Yin, XUE Shi-Wei, CHEN Gong-Xing, CHEN Shu-Lin. Establishment of the Transgenic Mice Expressing the HBV Receptor Human ASGPR[J]. China Biotechnology, 2010, 30(05): 87-91

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