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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志
中国生物工程杂志  2009, Vol. 29 Issue (11): 106-111    
综述     
乳酸菌载体pMG36e的应用现状
丁寅寅1,马会勤2,左芳雷1,郝彦玲1,陈尚武1**
1.中国农业大学食品科学与营养工程学院 北京 100083
2.中国农业大学农学与生物技术学院 北京 100193
Application of Lactic Acid Bacteria Vector pMG36e
DING Yin-yin1,MA Hui-qin2,ZUO Fang-lei 1,HAO Yan-ling1,CHEN Shang-wu1
1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2.College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China
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摘要：

乳酸乳球菌通用表达质粒pMG36e是一个经典的人工构建的组成型表达载体，是以乳酸乳球菌乳脂亚种蛋白酶基因的转录和翻译信号为基础构建而成的。它包含一个强启动子，能够在多种细菌中表达外源蛋白。已用于研究细菌素作用机制，乳酸菌基因工程菌株的改造以及口服疫苗的开发等，应用领域十分广泛，已成为乳酸菌基因工程研究的重要工具质粒之一。本文主要从载体构成、基因表达与食品级载体改造等三方面的应用对其进行综述，旨在为该质粒今后研究提供资料。
关键词： pMG36e;  表达载体;  食品级载体;  乳酸菌    
Abstract:

Plasmid pMG36e, based on protease gene transcription and translation signals from Lactococcus lactis cremoris, is a typical artificial constitutive expression vector as a main expression system in Lactococcus. It has a strong promoter and has been used for expression of heterologous protein such as enzyme in a variety of bacteria. In recent years, it was used to investigate the mechanism of bacteriocin as an intermediate vector, to construct lactic acid bacteria genetic strains and develop oral vaccine, even in gene therapy study. pMG36e has been used so widely in multi-disciplinary applications, it becomes one of the most important plasmid in the lactic acid bacteria genetic engineering. In this paper, the basic plasmid composition, structure, and application of pMG36e in gene expression and food-grade vector construction were reviewed to provide reference for future research.
Key words: pMG36e    expression vector    food-grade vector    vectorLactic acid bacteria
收稿日期: 2009-06-29 出版日期: 2009-12-07
ZTFLH:  Q782  
基金资助:

国家“863”计划资助项目（2008AA10Z310,2006AA10Z317）
通讯作者: 马会勤     E-mail: swchen@cau.edu.cn
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丁寅寅
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引用本文:

丁寅寅 马会勤 左芳雷 郝彦玲 陈尚武. 乳酸菌载体pMG36e的应用现状[J]. 中国生物工程杂志, 2009, 29(11): 106-111.

DING Yin-Yin, MA Hui-Qi, ZUO Fang-Lei, HAO Pan-Ling, CHEN Chang-Wu. Application of Lactic Acid Bacteria Vector pMG36e. China Biotechnology, 2009, 29(11): 106-111.

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https://manu60.magtech.com.cn/biotech/CN/    或    https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I11/106

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