中国生物工程杂志

Abstract Although BT insecticide had been widly applies in agricultural production , some problems still existed,like low yield and easier to lose activity.Polh protein that stable in natural circumstance is a protector for the virion. And more, the recombinant polyhedrin showed almost the same characteristics as the native baculoviral polyhedrin: it was rapidly solubilized under alkaline conditions and digested by alkaline proteases in the insect midgut. So our target is to investigate the use of the baculoviral Polh protein as a stable and protective fusion partner for the high expression of BT. Recombined BT cry3Aa with AcNPV Polyhedrin, construct the expression vector and made the recombined protein expressed in E.coli. Obtained the recombined protein engineering strain of cry3Aa and polyhedrin, and so laid foundation for the applied experimentation.