精原干细胞(Spermatogonial Stem Cells , SSCs)的体外培养是开展研究精原干细胞生物学的一项最基本的生物技术,近年来成为干细胞生物学研究的热点领域之一。如何获得高纯度、高活力的具有生物学安全性的SSCs是研究体外生物学的关键所在,而影响精原干细胞体外培养的因素很多,如动物种类和年龄、培养基、血清、饲养层的选择和生长因子等,长期以来一直认为精原干细胞无法在体外长期培养而且保持不分化,但随着研究的深入,尤其是鼠上,已经得到了SSCs体外长期培养。鼠作为研究哺乳动物甚至人常用的模拟生物,本文主要就近年来对鼠SSCs的研究现状及研究进展情况,并结合作者的工作经验,对体外长期培养鼠SSCs的影响因素作一详细评述。
In vitro culture of spermatogonial stem cells is a basic technology of biology ,which can promote the biology investigation of spermatogonial stem cells . So spermatogonial stem cells in vitro culture has become one of the hot fields in the Biology of the Stem Cell in recent years .Obtaining SSCs with high purity , plenty of vigour ,biological safty are the essential steps for SSCs related biological studies in vitro . However ,there are many factors affecting SSCs in vitro culture , such as animal species and age , culture medium , serum , selection of feeder layer and growth factors . SSCs always be thought not to culture in a long-term time in vitro ,which retain stem cell activity . But with the deeper and deeper investigation , people have already obtained the long-time culture of SSCs , specially in Murine . Murine is a direct investigation analogue organism to research mammalian even human in the end .This review mainly summarize the factors ,which affect SSCs in a long-term culture in vitro according to the recent related reports and our experimental work
张秀娟,张守全. 鼠精原干细胞体外培养影响因素的研究进展[J]. 中国生物工程杂志, 2008, 28(专刊): 187-190.
. The factors affect spermatogonial stem cells of Muine in vitro culture. China Biotechnology, 2008, 28(专刊): 187-190.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I专刊/187
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