中国生物工程杂志

The inclined gravitational settler showed some advantages in animal cells perfusion culture process. However, the further application of this settler had been limited because the cells were easy to deposit on its bottom. The properties of three-dimensional flow field inside the settler, which would influence the cells deposition, was investigated through the methods of combination of CFD technology and cold model experiments. The result showed that the amount of cells hung in the boundary layer were larger than that of adhered on the bottom. One of the alternative methods for this problem was to optimize settler structure by reducing the side-effect of boundary layer. With the result demonstrated that the amount of cells deposition could be reduced effectively in this optimized settler.