中国生物工程杂志

Abstract: To overcome the difficult of extraction RNA from Euonymus japonicus 'Cu zhi' tissue with high levels of phenolic compounds and carbohydrates, the study modified the SDS/phenol method, and isolated total RNA from the leaf and stem tissues of Euonymus japonicus 'Cu zhi'. The results showed that, the lightness of 28S rRNA in extracted total RNA was two times as much as that of 18S rRNA, the A260/A280 ration is 1.94, and the A260/A230 is 2.06.The yield of RNA was 252μg/g (gram fresh weight).The p5cs gene fragment(EU347715) was successfully amplified by RT-PCR suggested that, the total RNA extracted was of high integrality, high quality and high productivity. The total RNA isolated by this protocol is of sufficient quality for subsequent molecular applications.