中国生物工程杂志

We analyze the component of ion-exchange wastewater which can be produced in the process of manufacturing monosodium glutamate by this experiment, and attempt to use ion-exchange wastewater as the fermentation matrix to produce pullulan. The optimum conditions of culture are as follows: initial pH is 6.5,pullulan cells are transferred to 500ml conical flasks containing 150ml culture medium; inoculation is incubated according to 10% dosage; The culture medium is carried out in a rotary shaker incubator at 200rpm and at 28℃ for 7 days. As a result, 23.5g /L Crude pullulan can be obtained, and the conversion rate is 47%. By shucking off pigment through activated carbon column and then removing protein, the recovery rate of pullulan reaches 65%.