对裂褶菌所产的内切β-1,3-葡聚糖酶粗酶液通过饱和硫酸铵沉淀,DEAE-Sephadex A-50 柱层析和Sephadex G-75 柱层析进行纯化,纯化倍数为44.7倍,酶活回收率为11.6%。酶学性质研究表明,最适温度和pH分别为45℃和5.0,pH4.5-5.5范围内酶活相对稳定,热稳定性在60℃以下。Fe2+、Ba2+对该酶有激活作用,Zn2+、K+、Cu2+、Ag+、Hg2+、Ca2+对该酶有抑制作用。
The endo-β-1,3-glucanase from Schizophyllum commune Fr. was purified in three steps which comprised ammonium sulfate precipitation, DEAE-Sephadex A- 50 chromatography, and Sephadex G-100chromatography. The purified enzyme showed purification 14.60 fold, and an activity recovery of 11.8%. The optimal temperature and pH of the enzyme were 45 ℃ and 5 . 0 respectively . The β-1,3-glucanase was more stable at pH 4.5-5.5, and was relatively stable below 60 ℃. Zn2+、K+、Cu2+、Ag+、Hg2+、Ca2+had inhibitory effect on the enzyme activity; Zn2+, Ca2+, Fe2+ and Ba2+ could stimulate the activity
程维蓉,郑必胜,赵欣. 内切β-1,3-葡聚糖酶的分离纯化及性质研究[J]. 中国生物工程杂志, 2008, 28(专刊): 112-115.
. PURIFICATION AND PROPERTIES Of ENDO-β-1,3-GLUCANASE PRODUCED FROM SCHIZOPHYLLUM. China Biotechnology, 2008, 28(专刊): 112-115.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I专刊/112
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