八氢番茄红素合酶(Phytoene synthase ，PSY)是类胡萝卜素生物合成的限速酶，通过建立PSY基因的人参转化体系，可促进相应类胡萝卜素的合成，从而提高人参的营养价值。本研究以人参愈伤组织为受体，以PSY 为目的基因，应用根癌农杆菌介导法进行遗传转化。以抗性筛选人参受体转染效率为指标，从菌液浓度、侵染胞龄、侵染时间、共培养时间四方面优化了转化体系。进行了PCR、PCR-Southern和RT-PCR分析鉴定及β-胡萝卜素含量测定，初步证明外源基因PSY 已整合到人参的基因组中并在转录水平上进行了表达，β-胡萝卜素含量平均提高了26倍。该研究为改善和提高人参中类胡萝卜素含量提供了一种新的途径。
Phytoene synthase is a rate-limiting enzyme in the pathway of carotenoid biosynthesis. This paper is aimed at establishing a transformation of Ginseng callus cells, elevating the nutritive value through encouraging the composition of corresponding carotenoid. Taking Ginseng callus cells as acceptor, PSY gene were transformed into cells via Agrobacterium-mediated. PCR and RT-PCR analysis from the transferred gene plants proved that PSY gene has been transferred into Ginseng callus cells and can be expressed. The content of β-carotene was increased by an average of 26 times. This research established a base of improving and raising the contents of carotenoid in the Ginseng callus cell.
于源华,杜柏权,张勇,张丽. 八氢番茄红素合酶基因对人参的遗传转化[J]. 中国生物工程杂志, 2008, 28(2): 90-95.
. Genetic Transformation of Phytoene Synthase Gene into Ginseng Callus Cells. China Biotechnology, 2008, 28(2): 90-95.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I2/90
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