目的：建立一种能定量测定重组人睫状神经营养因子（ciliary neurotrophic factor，CNTF）生物学活性的新方法。方法：从鸡胚中分离出背根神经节并制成神经细胞，将重组人睫状神经营养因子加入到细胞中继续培养64h后，用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性，从而定量测定重组人睫状神经营养因子的生物活性。结果：重组人睫状神经营养因子有促原代鸡胚背根神经细胞存活作用，细胞存活率与加入重组人睫状神经营养因子的量成正相关。结论：通过检测存活的原代鸡胚背根神经细胞内酸性磷酸酶的含量来定量测定重组人睫状神经营养因子生物活性的实验方法具有干扰因素少、定量准确、重复性好等优点。
Object: To develop a new quantitative determination method for the biological activity of recombinant human ciliary neurotrophic factor. Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell，The rhCNTF was added to neuron cells and incubated for 64 hours，The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally. Result: rhCNTF could promote original era dorsal root neuron cells of chick embryo surviving，the livability of neuron cells was positively related to the amount of rhCNTF added to the culture. Conclusion: A quantitative determination method for the biological activity of rhCNTF was developed by testing the activity of acid phosphatase in neuron cells. Compared with the typical ways，this method was quantificational easily，repeatable better and with much fewer disturbance factors.
周虎,汪怡,朱俊铭. 定量测定重组人睫状神经营养因子活性的新方法[J]. 中国生物工程杂志, 2008, 28(2): 86-89.
. A New Quantitative Determination Method for The Biological Activity of Recombinant Human Ciliary Neurotrophic Factor. China Biotechnology, 2008, 28(2): 86-89.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I2/86
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