【目的】用活体骨髓瘤细胞SP2/0做融合提高融合率制备单克隆抗体，并与常规方法比较效果。【方法】将SP2/0细胞打到8周龄的SPF级BALB/c小鼠皮下，待实体瘤生长到直径达2～3cm时无菌解剖取实体瘤，分离出骨髓瘤细胞进行融合。同时用培养基培养SP2/0细胞进行融合做比较，分两组进行。比较两种方法的融合率以及两种方法制备出来的单克隆抗体的相对亲和力。【结果】做了6次融合，实体瘤融合组融合率为70.4％，常规法融合组44.6％，两种方法制备单抗的相对亲和力均达到1:100000以上。【结论】利用活体实体瘤细胞进行融合能明显提高细胞融合率。
【Objective】Prepare monoclonal antibody(MAb) using SP2/0 cell isolated from tumor in live mouse, and compare the efficiency of cell fusion with conventional method.【Methods】The SP2/0 cell were hypodermic injected in the back of the SPF level BALB/c mouse which were 8 week old. The SP2/0 cells were re-prepared by isolation from the tumor when it reached 2~3cm diameter. The fusion efficiency of SP2/0 cell from live tumor and from cell culture was compared by fusing with spleen cells. The relative affinity of MAbs made by two methods were also compared. 【Results】We have made six fusions. The fusion rate is 70.4% using SP2/0 cell from live tumor, while that is 44.6% using SP2/0 cell from cell culture. The relative affinity of MAbs made by two method were all above 1:100000. 【Conclusion】 We can significantly improve the fusion efficiency by fusion spleen cell with SP2/0 cell from live tumor.
国家“973”基金
李永亮,田美娜,卢曾军,杨苏珍,刘在新. 应用活体骨髓瘤细胞制备单克隆抗体[J]. 中国生物工程杂志, 2008, 28(11): 63-66.
https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I11/63
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