由胡萝卜软腐欧文氏菌胡萝卜软腐亚种 Erwinia carotovora subsp.carotovora （Ecc）引起的细菌性软腐病是世界性的重要流行病害，由于缺乏天然抗源，研究进展缓慢，拟南芥成为软腐病抗性研究的主要试材。本文选用 29 份拟南芥材料，制定了病情分级标准，以接种后 48 小时的病情指数作为软腐病抗性的鉴定指标，筛选出抗软腐病材料 CS906 和感病材料 CS20。通过分析抗、感材料接种 Ecc 后 AOS、ERF1-1、PR1、PDF1.2 和 PAL1 等 5 个基因的表达变化，发现 SA、ET 和 MJ 信号途径都参与了拟南芥对 Ecc 的防卫反应，且基因表达模式在抗、感材料中相似，差异体现在表达量上。本研究对深入探讨拟南芥软腐病抗病机制具有重要意义。
Soft rot caused by Erwinia carotovara.subsp.carotovora (Ecc) is a very serious disease in the world. Owing to the absence of natural resistant resources, the work moves slowly. Arabidopsis thaliana became the main target material for studying the mechanism of resistance to Ecc. In this paper, we selected 29 Arabidopsis accessions, then established the disease classification standards, and took the disease index at 48 hours post inoculated as the evaluation criteria of resistance to Ecc. The results showed that the ecotype CS906 was resistant and the ecotype CS20 was highly susceptive. By comparing gene expression changes of AOS, ERF1-1, PR1, PDF1.2 and PAL1 between the resistant and susceptible accessions at different time points after inoculation, we found that SA、ET and MJ signal transduction pathways were involved in the defense response of Arabidopsis to Ecc, and gene expression model was similar in CS906 and CS20, but the expression levels of genes in CS906 were higher than that in CS20. Upper results will facilitate the study on the resistance mechanism of Arabidopsis to Ecc.
省部基金;国家“863”计划资助项目
叶雪凌,曹鸣庆,马荣才. 拟南芥对细菌性软腐病抗性变异分析[J]. 中国生物工程杂志, 2008, 28(11): 36-42.
https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I11/36
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