中国生物工程杂志

In this article , rapid propagation of Panax ginseng in tissue culture was reported ; the process of morphogenesis through different path from shoots , stems , leaves and seeds as explants obtained regenerated plant . The callus was induced on MS medium supplemented with 4.0mg/L 2,4-D and 0.2mg/L BA .The callus was subcultured on MS medium supplemented with 2.0mg/L 2,4-D and 0.2mg/L KT. Embryogenic callus could be obtained and then lacking 2,4-D we can obtain embryoid. It can grow fast on MS medium with no growth regulators. Plantlets regenerated on 1/2MS. We assume that the origin of somatic embryo of Panax ginseng is from the single-cell. The origin of adventitious buds of Panax ginseng is from callus surface or inner. High sugar and starch involve in its embryoid callus and development. High activities of POD and PPO and protein level were necessary to earlier offer material and energy. The contents of IAA is higher in earlier embryo and ABA is higher in mature embryo. The ratios of ABA/IAA were the development of somatic embryogenesis.