红谷霉素是链霉菌702发酵所产的一种生物活性物质,具有较强抗细菌活性。在摇瓶条件下,对链霉菌702生料发酵生产红谷霉素进行研究。采用单因素实验探索生料发酵工艺,筛选出摇瓶生料发酵条件为7.5%的种子接种量和添加抗菌剂1.5ml。在此条件下进行生料发酵,使红谷霉素摇瓶发酵产量达到1.46g/L,比常规灭菌发酵产量提高25.7%,实验结果表明红谷霉素生料发酵是一种潜在可行的发酵方法,不但可以很好的节约发酵成本,还可以提高发酵产量。
Honggumycin, which showed a significant antibacterial activity, was a bioactive compound obtained by the fermentation broth of Streptomyces 702. Under the shaking-flask culture, unsterile fermentation process of honggumycin produced by Streptomyces 702 were studied by single-factor experiment design. The optimum unsterile shaking-flask cultivation condition consisted of: 7.5% of inoculation size (v/v), 1.5mL of antimicrobial agent addition. Under the optimal conditions, honggumycin yield of unsterile fermentation was up to 1.46g/L, which was increased by 25.7% than that of sterile fermentation. The result showed that fermentation for Streptomyces 702 under non-sterile conditions was an potentially efficient fermentation mode, which not only saved fermentation cost, but also increased fermentation productivity.
熊智强,涂晓嵘,涂国全. 红谷霉素生料发酵工艺的研究[J]. 中国生物工程杂志, 2008, 28(1): 76-79.
. Studies on Unsterile Fermentation Process of Honggumycin. China Biotechnology, 2008, 28(1): 76-79.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2008/V28/I1/76
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