大肠杆菌表达系统是基因表达技术中发展最早和目前应用最广的经典表达系统。利用该系统表达重组蛋白具有许多优越性,但其表达效率受诸多因素的影响。本文综述国内外利用大肠杆菌表达系统高效表达外源蛋白的策略,主要包括选择合适的启动子、改变信号肽结构、提高mRNA稳定性、提高翻译效率、表达稀有密码子、降低包涵体形成及蛋白降解,利用融合蛋白与分子伴侣、调控发酵条件实现高密度培养等。
Cloning and expression of recombinant protein in Escherichia coli has been developed for a long time and it is widely used as a classical expression system now. Many advantages of this system have been confirmed, however, disadvantages also exist. The efficiency of expression is affected by many factors. This paper summarized strategies for high-level expression of recombinant protein in this system. These strategies mainly include selection of appropriate promoter, optimization of signal peptide, increasing the stability of mRNA and the efficiency of translation, preventing the use of low usage codons, reducing inclusion body formation and degradation of the target protein, using molecular chaperones and fusion protein, development of high-cell-density fermentation, and etc.
任增亮,堵国成,陈坚,吴敬. 大肠杆菌高效表达重组蛋白策略[J]. 中国生物工程杂志, 2007, 27(9): 103-109.
Jian Chen . Strategies for high-level expression of recombinant protein in Escherichia coli. China Biotechnology, 2007, 27(9): 103-109.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I9/103
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