为寻找胃癌特异的肿瘤标记物，用于胃癌临床诊断及药物治疗靶点的选择，本研究采用荧光差异显示凝胶电泳（DIGE）技术分离并筛选 Cy3、Cy5 及 Cy2 荧光素标记的胃癌及对应癌旁组织差异表达蛋白质，用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）或串联质谱技术进行鉴定并分析。结果共筛选出 33 个差异表达蛋白质点，其中 9 个蛋白质点在胃癌组织中上调，24 个蛋白质点下调。对 22 个蛋白质点采用质谱技术成功鉴定，突变结蛋白、锰超氧化物歧化酶、热休克蛋白 60等在胃癌中高表达，热休克蛋白 27、前列腺素 F 合酶、硒结合蛋白 1、锌指蛋白 160、微管蛋白 α6、真核生物翻译延伸因子 1 α1 等在胃癌组织中低表达，并筛选出 5 个未知蛋白。这些差异表达蛋白可望成为胃癌诊断的特异标记物，并与胃癌的发生、发展及预后等有关，为胃癌的诊断、发生机制的研究提供了新的思路。

To identify specific protein markers for gastric cancer detection and diagnosis, as well as develop new potential therapeutic targets of the disease. Gastric cancer tissues and adjacent normal mucosa were examined by the fluorescence differential in-gel electrophoresis (DIGE) technique after labeled with CyDye DIGE fluors Cy3, Cy5 and Cy2. Protein spots detected differential with statistical significance were identified by MALDI-TOF MS or MS/MS. As the result, intensity changes of 33 spots were detected with statistical significance. 9 protein spots of them up-regulated otherwise 24 down-regulated in gastric cancer tissues. And 22 of them were identified by MALDI-TOF MS or MS/MS successfully. Several proteins up-regulated such as MnSOD, HSP60, mutant desmin et al. HSP27, prostaglandin F synthase, SeBP 1, zinc finger protein 160, tubulin alpha 6, eTEF1 A 1 and so on were down-regulated. Our results suggest that DIGE is a useful technique for differential expressed proteins screening and analysis in gastric cancer tissues which may be useful for the development of new molecular markers for diagnosis and prognosis of gastric carcinoma. This differently expressed proteins may be useful tumor markers for gastric cancer.