为研究转基因烟草中产生西红花酸的可行性，在本研究中，西红花玉米黄素裂解酶（CSzCD）基因插入到pBI121载体的花椰菜花病毒（CaMV）35S启动子下游，通过农杆菌介导整合到烟草基因组中。通过Southern blotting 分析得到21株转基因烟草植株系; 转基因烟草叶片提取物Western blotting 和HPLC分析显示有西红花酸的产生，然而阴性对照中并没有发现西红花酸的存在。
To investigatethe feasibility to produce crocetin in tobacco plants. In this study, the coding region of zeaxanthin cleavage dioxygenase (CsZCD) gene from crocus sativus L. was inserted into the downstream of the cauliflower mosaic virus (CaMV) 35S promoter of a binary vector pBI121, and integrated into the genome of Nicotiana tabacum L. Twenty-one transgenic lines were identified by genomic southern blotting analysis. Western blotting and HPLC analysis of the leaf extracts of transgenic tobacco showed that crocetin was produced in CsZCD gene-transformed plants, while no crocetin was found in the untransformed tobacco.
肖海波,王岚,严琛,徐莺,陈放,唐琳. 转基因烟草表达西红花CSzCD基因产生西红花酸[J]. 中国生物工程杂志, 2007, 27(6): 51-55.
. Transgenic tobacco expressing the crocus CsZCD gene showed the production of crocetin. China Biotechnology, 2007, 27(6): 51-55.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I6/51
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