了解牛睾丸支持细胞体外培养的生物学特性，试验采用组合酶消化和选择贴壁法，将5月龄、6月龄牛胎儿及新生牛睾丸支持细胞分离纯化后进行体外培养。试验结果显示，这一阶段牛睾丸适宜支持细胞分离纯化用；采用0.25 %胰蛋白酶+0.02 %EDTA二次消化法是一种经济有效的牛胎儿睾丸支持细胞分离方案；试验发现，牛胎儿睾丸支持细胞体外研究时间应控制在3天～20天内，处于对数生长期的牛胎儿睾丸支持细胞，对牛体外受精卵体外发育有明显的促进作用。
In order to study the growth behavior of bovine Sertoli cells, adopting the combination of enzymatic digestion and different attaching velocities methods to isolate and purify Sertoli cells of 5-month, 6-month bovine fetal testis and newborn bovine testis, then cultured in vitro. Indicating that bovine testis in this stage were suitable for isolating and purifying Sertoli cells, 0.25% trypsin+0.02%EDTA is an economic and efficient method to isolate bovine fetal Sertoli cells, and the optimal research period of bovine fetal Sertoli cells should be controlled within 3-20 days, bovine fetal Sertoli cells in exponential growth stage can obviously promote the development of bovine IVF zygotes in vitro.
赵云程,许琴,刘赛,陈静波. 牛睾丸支持细胞分离、纯化及体外生长行为的研究[J]. 中国生物工程杂志, 2007, 27(5): 96-100.
. The Study of Isolation , Purification and Growth Behavior of Bovine Sertoli Cells. China Biotechnology, 2007, 27(5): 96-100.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I5/96
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