重组PCR是用聚合酶链反应体系来实现DNA重组的技术。经过二十多年的发展,该技术已形成三个各具特色的方法分支:重叠延伸拼接、跳跃PCR和DNA重排。近几年,以利用天然的差异基因作为重组来源为目的,通过简化操作步骤、提高捕获变异的效率、借助于表面展示技术和荧光探针技术搭建的高通量筛选平台,重组PCR技术在基础研究和工程应用研究的众多领域中的应用价值不断增加。
Recombinant PCR applies to fulfill gene recombination by PCR thermal reaction. Over the twenty years, it has branched into three characteristic strategies: splicing by overlapping extension (SOE), jumping polymerase chain reaction (JPCR) and DNA shuffling. Recently, the technique aimed with exploiting natural source of different allele genes is developing up on simplification of experimental procedure, on trap for mutation and variation, and on high-throughput screening with technology of surface display and fluorescent probe. The recombinant PCR is increasesing value in broad range from biological basic research to bioengineering study.
王皓,康现江. 重组PCR技术研究进展和应用[J]. 中国生物工程杂志, 2007, 27(5): 153-156.
. The Progress and Application of Recombinant PCR. China Biotechnology, 2007, 27(5): 153-156.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I5/153
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