目前双基因和多基因转基因植物已经商品化,并展现了广泛的应用前景。但在转基因植物研究中,使多个基因同时在植物体中表达调控依然很难实现,是植物基因工程和生物技术发展中的难点。融合基因表达载体作为一种新型的方法,弥补了获得双价或多价转基因植物传统方法的缺点,具有更高的应用价值。本文对目前构建融合基因的方法作了评述,并对比较新颖的连接肽2A和LP4做了详细介绍。
Transgenic plants expressing two or more novel foreign genes have been commercialized and have shown broad perspective. However,one of the major technical hurdles impeding the advance of plant genetic engineering and biotechnology is the fact that the expression or manipulation of multiple genes in plants is still difficult to achieve. A novel expression system of coordinating the expression of multiple protein in a single open reading frame overcome the drawbacks of the conventional techniques which already exist for the stacking of genes and traits in transgenic plants. It is a feasible method for multiple genes transformation. In this article, we discussed the conventional technique introducing multiple genes into plants and the novel polyprotein expression system for transgenic research; and especially described more details about the novel linker peptide 2A and LP4 which were used to connect different protein sequence into one polyprotein.
李艳萍,郎志宏,李敏,李国勋,黄大昉. 融合基因在转基因植物中的应用[J]. 中国生物工程杂志, 2007, 27(5): 137-141.
. The application of polyprotein in transgenic plants. China Biotechnology, 2007, 27(5): 137-141.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I5/137
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