以获得大量胞外青霉素酶为目的，将青霉素酶基因克隆至表达载体pWB980中，并转化到双蛋白酶缺陷的Bacillus subtilis DB104。重组菌在LB培养基中培养24小时后， SDS-PAGE分析发现目的蛋白分子量为28kDa，酶活力为339U/mL；通过筛选7种不同的发酵培养基发现4#培养基更利于青霉素酶的表达，最大酶活力为1580U/mL，较优化前提高了3.66倍，并对该重组菌进行了7L罐放大实验，结果显示在培养24小时产酶达到高峰，酶活力为1255.8 U/mL。
To obtain a number of extracellular penicillinase, the gene (penp) encoding penicillinse of Bacillus cereus ATCC10987 was cloned into expression vector in Bacillus subtilis , transformed into Bacillus subtilis DB104 deficient in tow proteases。When recombinant bacteria was cultured in LB medium for 24 hours, the result of SDS-PAGE showed that the molecular weight of the protein was 28KDa and the penicillinase activity reached 339U/ml; By screening severn different fermentation media, the result showed that 4# medium is favorable to producing penicillinase by the recombinant cells than the others, with the yield of the enzyme being 1580U/mL; when the recombinant cells was cultured in 7 liter fermentor for 24h ,the penicillinase activity reached 1255.8U/ml．
赵洪坤,刘兴旺,邱强,李秀星,杜连祥. 青霉素酶基因在枯草芽孢杆菌中的高效表达[J]. 中国生物工程杂志, 2007, 27(12): 31-35.
. High-Level Expression of Penicillinase Gene in Bacillus Subtilis. China Biotechnology, 2007, 27(12): 31-35.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2007/V27/I12/31
Cited
