将猪细小病毒VP2基因克隆至pCI-neo真核表达载体中，构建了pCIneo-VP2重组质粒，转染至PK-15细胞中，利用免疫荧光方法检测在体外表达情况；并以小鼠为动物模型，将pCIneo-VP2、pCI-neo重组质粒、猪细小病毒活疫苗和对照组通过肌肉注射进行免疫，检测免疫小鼠的淋巴细胞转化功能，特异性CTL杀伤活性和血清抗体滴度。结果显示，pCIneo-VP2在体外能够诱导PK-15细胞表达VP2蛋白，小鼠注射pCIneo-VP2质粒 1周后能够诱导机体产生抗体，4周时达到峰值，与活疫苗对照组产生的抗体滴度、诱导T淋巴细胞增殖和诱导强的细胞毒性基本一致。试验表明，构建的pCIneo-VP2能够有效诱导机体产生体液免疫和细胞免疫，为研制出高效、新型猪细小病毒疫苗提供了科学依据和试验依据。

VP2 gene of Porcine Parvovirus (PPV) was inserted into eukaryotic expression vector. Then the eukaryotic expression vector, PCIneo-VP2 plasmids was constructed. The plasmid was transfected to PK-15 cells. The expressed VP2 protein were detected by direct fluorescentantibody in vitro. Inoculated mice with PCIneo-VP2 by muscle injection and detected antibody of PPV by competitive ELISA. And in order to study immune effect of nucleotide vaccine, we inspected the invert function of lymphocyte and the cytotoxic activity by MTT. The results suggested that the mice inoculated with PCIneo-VP2 can produce antibodies after a week. At the fourth week, the level of antibodies grew to peak. These have a coincidence with the control groups injected with live vaccines. By lymphocyte growth test, we found that PCIneo-VP-2 and live vaccines could induce the increase of T-lymphocyte, but the increase of B-lymphocyte couldn’t be induced, and they could inhance the cell toxicity. The experiment proved that the humoral immunity and cellular immunity could be induced efficiently by PCIneo-VP2.