川草2号老芒麦（Elymus sibiricus L.）UV-B辐射敏感基因rbcL的克隆及其调控表达研究

中国生物工程杂志

研究报告

川草2号老芒麦（Elymus sibiricus L.）UV-B辐射敏感基因rbcL的克隆及其调控表达研究

何文兴李洪梅徐莺肖猛李群颜钫王胜华陈放

济南大学济南大学四川大学四川大学四川大学四川大学四川大学四川大学

Isolation of the Genes Related to UV-B Radiation Ensitivity

全文: PDF HTML

摘要： rbcL是编码光合关键酶1,5-二磷酸核酮糖羧化酶（Rubisco）大亚基的基因。本文运用mRNA差异显示技术（DDRT-PCR）并通过5ˊRACE (Rapid Amplification of cDNA Ends) 从高原植物川草2号老芒麦（Elymus sibiricus L. cv. 'chuancao No.2'）中获得了受增强UV-B辐射抑制的rbcL基因，该基因全长cDNA为1.51kb，开放阅读框（ORF）长1.434kb，编码477个氨基酸。氨基酸序列与Elymus trachycaulus中的Rubisco大亚基具有97％的同源性、与Triticum aestivum和Hordeum comosum的Rubisco大亚基同源性均为 98％。Northern杂交分析表明，增强UV-B辐射后6h，rbcL基因表达受到强烈抑制，处理后60h，其表达几乎完全被抑制，表明即使是长期生长在高原地区、强UV-B辐射条件下的高原物种，在受到较强的UV-B辐射后，其rbcL基因的转录也会受到抑制。

Abstract: rbcL is the gene which codes the large subunit of the photosynthetic enzyme, Rubisco. Its EST sequence, whose expression is inhibited by the enhanced UV-B radiation, was obtained from the leaf cDNA of alpine plant Elymus sibiricus L. cv. 'chuancao No.2' by reverse transcription-polymerase chain reaction (RT-PCR) differential display method and a 1.51 kb full-length cDNA was cloned through 5' RACE. The rbcL has a 1.434 kb open reading frame (ORF) which encodes a precursor protein of 477 amino acid residues. The amino acid sequence exhibits 97%, 98% and 98% positional identity with its correspondent from Elymus trachycaulus、Triticum aestivum、Hordeum comosum, respectively. Northern hybridization analysis on the rbcL expression during the time of enhanced UV-B radiation, showed that the mRNA level of the rbcL descended obviously after being exposed to enhanced UV-B radiation for 6h, and 60h later, the mRNA even couldn't be detected. 'chuancao No.2' Growing in the plateau area for a long time and being radiated strongly by the UV-B, the rbcL transcription of Elymus sibiricus L might be restrained like the plain plants, which are sensitive to UV-B.

收稿日期: 2006-07-31 出版日期: 2006-12-25

通讯作者: 何文兴

	服务
	把本文推荐给朋友
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	李洪梅
	肖猛
	颜钫
	李群
	徐莺
	何文兴
	王胜华
	陈放

引用本文:

何文兴,李洪梅,徐莺,肖猛,李群,颜钫,王胜华,陈放. 川草2号老芒麦（Elymus sibiricus L.）UV-B辐射敏感基因rbcL的克隆及其调控表达研究[J]. 中国生物工程杂志, .

. Isolation of the Genes Related to UV-B Radiation Ensitivity. China Biotechnology, .

链接本文:

https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I12/56

No related articles found!

Viewed

Full text

Abstract

Cited

Shared

Discussed