双水相体系逆流色谱技术在蛋白质分离中的应用

中国生物工程杂志

综述

双水相体系逆流色谱技术在蛋白质分离中的应用

李挺曹学丽董银卯

北京工商大学化学与环境工程学院北京工商大学化学与环境工程学院北京工商大学化学与环境工程学院

The Application of Countercurrent Chromatography with Aqueous Two Phase System in the Separation of Proteins

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摘要： 双水相体系逆流色谱技术结合了逆流色谱的高效率、高制备量以及双水相体系适于蛋白质分离的特点，因此在蛋白质的分离方面具有独特的应用价值。本文综述了近年来基于正交轴逆流色谱仪器的双水相体系逆流色谱技术在多种蛋白质分离中的应用。并对一些新兴的蛋白质逆流色谱分离技术及新型逆流色谱柱分离系统进行了介绍。

Abstract: Aqueous two phase system(ATPS) provides a gentle, non-denaturing separation environment for proteins, enzymes. While high-speed countercurrent chromatography (HSCCC) is a liquid-liquid partition chromatography which uses centrifugal force to hold the stationary phase and facilities the mobile phase partitioning through the stationary phase, it can produce high separation efficiency with large sample loading capacity. However, the ordinary HSCCC apparatus (Type J ) fails to retention a satisfactory stationary phase of ATPS because of its high viscosity and low interfacial tension. Nevertheless, the later designed cross-axis planetary centrifuge system(X-CPC) can produce a greater lateral force field and enhances significantly the retention of the ATPS stationary phase. This paper gives a review of the application of these CCC techniques with ATPS in the separation of proteins. Meanwhile, new techniques such as pH－peak focusing CCC and dye-ligand affinity CCC and some new CCC column design for improvement of separation efficiency and retention of ATPS stationary phase are introduced in this paper.

收稿日期: 2005-09-15 出版日期: 2006-02-25

通讯作者: 曹学丽

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引用本文:

李挺,曹学丽,董银卯. 双水相体系逆流色谱技术在蛋白质分离中的应用[J]. 中国生物工程杂志, .

. The Application of Countercurrent Chromatography with Aqueous Two Phase System in the Separation of Proteins. China Biotechnology, .

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I02/83

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