毛白杨纤维素合成酶基因（PtoCesA1）克隆及正义植物表达载体的构建

中国生物工程杂志

研究报告

毛白杨纤维素合成酶基因（PtoCesA1）克隆及正义植物表达载体的构建

李春秀齐力旺王建华张守攻

中国林业科学研究院林业科学研究所中国林业科学研究院林业科学研究所中国林业科学研究院林业科学研究所

Cloning of a Cellulose Synthase Gene from Aspen PtoCesA1 and Construction of it's Plant Expresstion Vector

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摘要： 以毛白杨形成层为材料克隆了毛白杨纤维素合成酶基因（PtoCesA1），序列分析表明该基因序列为3215bp, 与欧洲颤杨的PtCesA1基因同源性为97% 具有开放的阅读框，编码区在52~2985碱基之间，编码区为2925bp。通过同义突变引入BamHI酶切位点，将全长基因克隆到植物表达载体pBI121中，经酶切和PCR鉴定确认载体构建正确，为下一步ptoCesA1转基因功能研究打下了基础。

Abstract: The DNA fragment was cloned from the cDNA that was synthesized using the RNA from Chinese white poplar and verified by sequencing This cDNA clone ,designated PtoCesA1, was 3215 bp long with an opening reading frame of 2934bp extending from nucleotides 52~2985.Comprision of the nucleotide sequence of PtoCesA1 with PtrCesA1 showed 97% identity.For construction of the PtoCesA1 binary expression vector, the BamHI site was made by synonymy mutation, the full length PtoCesA1 cDNA was subcloned into pBI121 between the BamHI site and XbaI site. The PtoCesA1 binary vector，containing PtoCesA1,was verified by digestion and PCR.

收稿日期: 2006-02-09 出版日期: 2006-02-25

通讯作者: 张守攻

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引用本文:

李春秀,齐力旺,王建华,张守攻. 毛白杨纤维素合成酶基因（PtoCesA1）克隆及正义植物表达载体的构建[J]. 中国生物工程杂志, .

. Cloning of a Cellulose Synthase Gene from Aspen PtoCesA1 and Construction of it's Plant Expresstion Vector. China Biotechnology, .

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I02/49

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