LFA-1基因敲出鼠胶原诱导风湿性关节炎发病率及程度的降低

中国生物工程杂志

研究报告

LFA-1基因敲出鼠胶原诱导风湿性关节炎发病率及程度的降低

王轶楠王世瑶柳忠辉崔雪玲台桂香

吉林大学基础医学院免疫教研室吉林大学基础医学院免疫教研室吉林大学基础医学院免疫教研室吉林大学基础医学院免疫教研室吉林大学基础医学院免疫教研室

Reduced incidence and severity of collagen-induced arthritis in mice lacking LFA-1

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摘要： 淋巴细胞功能相关抗原（LFA-1）属于黏附分子家族，在细胞相互作用中发挥重要作用。通过观察LFA-1基因敲除小鼠的胶原诱导关节炎(CIA)发病率、放射线学及组织学变化，探讨LFA-1分子在CIA发病中的作用。实验采用100μg的二型胶原(CII)加弗氏完全佐剂充分混合，在LFA-1基因敲除小鼠及正常对照组小鼠尾根部皮内注射，21天后重复免疫一次，随后进行发病率、放射线学及组织学分析。研究结果显示，CII免疫后，正常对照组小鼠平均发病率为57%，临床可见明显的放射线及组织学的异常变化。而LFA-1基因敲除小鼠无发病，放射线及组织学检查亦无明显异常改变。上述结果提示LFA-1在胶原诱导的关节炎发病中起重要作用。

Abstract: Lymphocyte function associated antigen1 (LFA-1) is a member of integrin family, that plays an important role in the adhesion of lymphocytes with other cells and matrix. To investigate the role of LFA1 in collageninduced arthritis (CIA), the incidence of CIA, histological and radiological assessments in the LFA-1 deficient (LFA-1 -/-) mice and control mice were examined. LFA-1 -/- mice and control mice were immunized with 100μg collagen type II(CII) emulsified with an equal volume of Freund’s complete adjuvant (CFA), followed by the booster injection of the same amount of CII in CFA on day 21. Then, clinical, histological and radiological assessments were done. It showed that 57% control mice developed arthritis and apparently changed in the histological and radiological assessment, whereas the all of LFA-1-/-mice had the normal histological and radiographic response and none developed arthritis. These results suggeste that LFA1 is indispensable for the onset of CIA.

收稿日期: 2005-11-18 出版日期: 2006-02-25

通讯作者: 柳忠辉

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引用本文:

王轶楠,王世瑶,柳忠辉,崔雪玲,台桂香. LFA-1基因敲出鼠胶原诱导风湿性关节炎发病率及程度的降低[J]. 中国生物工程杂志, .

. Reduced incidence and severity of collagen-induced arthritis in mice lacking LFA-1. China Biotechnology, .

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https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I02/34

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