摘要：从板栗中分离出两个新的MADS-box基因片段，通过3’RACE方法获得了基因全长，命名为CmMADS2(DQ148294) 和 CmMADS4(DQ148296)。CmMADS2推导的氨基酸序列有242个,含56个氨基酸的MADS-box区。CmMADS4推导的氨基酸序列也有242个,含72个氨基酸的MADS-box区。RT-PCR分析结果显示，CmMADS2基因在板栗的带花叶和花序中有表达，在其它组织中（不带花嫩叶﹑老叶﹑茎﹑果实）不表达。而CmMADS4基因在板栗的所有组织中（带花叶﹑花序﹑不带花嫩叶﹑老叶﹑茎﹑果实）都表达。结果表明这两个基因可能参与板栗花器官发育的调控
Abstract: we cloned two completely new MADS-box genes from Castanea mollissima by the method of 3’RACE, named CmMADS2(DQ148294) and CmMADS4(DQ148296) respectively. CmMADS2 encoded 242 identical amino acids (aa) including 56 aa of the MADS-box domain and CmMADS4 encoded 242 identical amino acids (aa) including 72 aa of the MADS-box domain. RT-PCR analysis showed that CmMADS2 expressed in inflorescences and tender leaves with flowers, not in other tissue(fruit﹑old leaves﹑stem and tender leaves with no flowers). While CmMADS4 was expressed in all the tissue mentioned above, suggesting that these novel MADS-box genes may be involved in floral organ development of Castanea mollissima.
刘涛,李晓贤,周浙昆,胡运乾. 板栗(Castanea mollissima) 中两个MADS-box基因的克隆和表达分析[J]. 中国生物工程杂志, 2006, 26(0): 76-78.
. Cloning and expression of two MADS-box genes from Castanea mollissima. China Biotechnology, 2006, 26(0): 76-78.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/76
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