细胞周期蛋白依赖性激酶抑制剂(ICK)是一种能够影响细胞生长和发育的重要调控蛋白。本文在大肠杆菌BL21中表达了从水稻9311中获得的水稻细胞周期蛋白依赖性激酶抑制剂基因OsICK1，并用表达蛋白免疫小鼠；Western blotting 结果表明得到的多克隆抗体不仅能够与诱导表达的OsICK1蛋白特异性结合，而且能够与水稻中的ICK蛋白特异性的结合，为研究水稻中OsICK1基因的表达奠定了基础。
Rice cyclin-dependent kinase inhibitor(ICK)is a key control protein in cell-cycle, it can effect the growth and development of cell. Rice OsICK1 gene was expressed in BL21,with the prokaryotic expression vector pET-28a(+).And anti- OsICK1 polyclonal antibody was obtained by injecting OsICK1 into mouse. The result of Western blotting show that the polyclonal antibody not only can differentially combine with the expressed protein OsICK1, but also can differentially combine with the OsICK1 in rice.It have established the base for the research of OsICK1 gene expression in rice.
何艺宾,王蕾,曾雅明,沈明山,陈亮. 水稻OsICK1基因的原核表达及其抗体制备[J]. 中国生物工程杂志, 2006, 26(0): 61-64.
. Prokaryotic expression of OsICK1 gene and anti-OsICK1 antibody preparation. China Biotechnology, 2006, 26(0): 61-64.
https://manu60.magtech.com.cn/biotech/CN/ 或 https://manu60.magtech.com.cn/biotech/CN/Y2006/V26/I0/61
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